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Sci Data. 2019 Feb 26;6:190025. doi: 10.1038/sdata.2019.25.

Map of physical interactions between extracellular domains of Arabidopsis leucine-rich repeat receptor kinases.

Author information

1
Department of Biology, University of Toronto - Scarborough, 1265 Military Trail, Toronto, Ontario, Canada.
2
Department of Cell & Systems Biology, University of Toronto, 25 Willcocks St., Toronto, Ontario, Canada.
3
Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr Bohr Gasse 3, Vienna, 1030, Austria.
4
Department of Biology, University of Alabama at Birmingham, Birmingham, Alabama, USA.
5
Protein Technologies Facility, Vienna Biocenter Core Facilities (VBCF), Vienna, Austria.
6
Centre for the Analysis of Genome Evolution & Function, 25 Willcocks St., University of Toronto, Toronto, Ontario, Canada.

Abstract

Plants use surface receptors to perceive information about many aspects of their local environment. These receptors physically interact to form both steady state and signalling competent complexes. The signalling events downstream of receptor activation impact both plant developmental and immune responses. Here, we present a comprehensive study of the physical interactions between the extracellular domains of leucine-rich repeat receptor kinases (LRR-RKs) in Arabidopsis. Using a sensitized assay, we tested reciprocal interactions among 200 of the 225 Arabidopsis LRR-RKs for a total search space of 40,000 interactions. Applying a stringent statistical cut-off and requiring that interactions performed well in both bait-prey and prey-bait orientations resulted in a high-confidence set of 567 bidirectional interactions. Additionally, we identified a total of 2,586 unidirectional interactions, which passed our stringent statistical cut-off in only one orientation. These datasets will guide further investigation into the regulatory roles of LRR-RKs in plant developmental and immune signalling decisions.

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