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Sheng Wu Gong Cheng Xue Bao. 2019 Feb 25;35(2):298-306. doi: 10.13345/j.cjb.180141.

[Optimization of noni callus induction and establishment of callus suspension system].

[Article in Chinese; Abstract available in Chinese from the publisher]

Author information

1
Southwest Landscape Architecture Engineering Research Center of State Forestry Administration, College of Horticulture and Gardening, Southwest Forestry University, Kunming 650224, Yunnan, China.
2
Southwest Institute of Green Development, Kunming 650224, Yunnan, China.

Abstract

in English, Chinese

The aim of the study was to obtain the secondary metabolites in the stem segment of noni and to establish genetic transformation system. The stem segments (no axillary buds) of noni were used as explants to induce the callus, and then to establish the cell suspension system. The factors affecting callus induction and cell suspension were studied. The results showed that the optimal culture medium for induction was MS with 1.0 mg/L 6-Benzylaminopurine (6-BA) and 0.1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), and the optimum culture medium for suspension was MS with 1.0 mg/L 6-BA and 0.1 mg/L 2,4-D, 3% sucrose and the pH of 5.85, with the initial inoculation amount of 37.5 g/L, and the speed of 110 r/min and 25±2 °C applying darkness culture. The suspension cells grew well and showed the maximum growth rate. The growth curve of the suspension cells from the stem segment of noni was in "S-typed" trend, and it should be transformed to the fresh medium between 12 and 20 d. During the culture, the pH of the culture medium decreased and then slowly increased, and the optimum pH for the suspension cells culture of callus from noni's stem segments was 4.5-5.0. In this study, the stable cell suspension system of the stem segment of noni was successfully established.

KEYWORDS:

Morinda citrifolia Linn.; callus; growth curve; stem; suspension culture

PMID:
30806060
DOI:
10.13345/j.cjb.180141
[Indexed for MEDLINE]
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