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Nat Methods. 2019 Mar;16(3):235-238. doi: 10.1038/s41592-019-0327-9. Epub 2019 Feb 25.

Universal light-sheet generation with field synthesis.

Author information

1
Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX, USA.
2
Department of Biophysics, UT Southwestern Medical Center, Dallas, TX, USA.
3
Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
4
Lyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, TX, USA.
5
Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX, USA. Reto.Fiolka@utsouthwestern.edu.
6
Lyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, TX, USA. Reto.Fiolka@utsouthwestern.edu.

Abstract

We introduce field synthesis, a theorem and method that can be used to synthesize any scanned or dithered light sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. Compared to LLSM, this user-friendly and modular approach offers a simplified optical design, higher light throughput and simultaneous multicolor illumination. Further, field synthesis achieves lower rates of photobleaching than light sheets generated by lateral beam scanning.

PMID:
30804550
PMCID:
PMC6561754
DOI:
10.1038/s41592-019-0327-9
[Indexed for MEDLINE]
Free PMC Article

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