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3 Biotech. 2019 Mar;9(3):88. doi: 10.1007/s13205-019-1634-7. Epub 2019 Feb 15.

Astaxanthin supplementation reduces dichlorvos-induced cytotoxicity in Saccharomyces cerevisiae.

Author information

1
Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry, 605014 India.

Abstract

This study evaluates the protective effect of astaxanthin against dichlorvos cytotoxicity in yeast Saccharomyces cerevisiae. Dichlorvos induce a dose-dependent cytotoxicity in yeast cells, which is mediated by oxidative stress. Our experimental results showed pre-treatment with astaxanthin enhances cell viability by 20-30% in yeast cells exposed to dichlorvos. A decrease in DCF fluorescence intensity and lipid peroxidation, increased SOD activity, and glutathione levels in astaxanthin-treated cells indicate that astaxanthin protected the cells against dichlorvos-induced oxidative stress. Reduced chromatin condensation and nuclear fragmentation in astaxanthin pre-treated cells also indicate that astaxanthin rescued the cells from dichlorvos-induced apoptosis. Our overall results suggest that dichlorvos induces oxidative stress-mediated cytotoxicity in yeast cells, and that was rescued by astaxanthin pre-treatment.

KEYWORDS:

Apoptosis; Astaxanthin; Cytotoxicity; Dichlorvos; ROS; Yeast

PMID:
30800599
PMCID:
PMC6385069
[Available on 2020-03-01]
DOI:
10.1007/s13205-019-1634-7

Conflict of interest statement

Compliance with ethical standardsThe authors declare that they have no competing interest.

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