Format

Send to

Choose Destination
Mol Imaging. 2019 Jan-Dec;18:1536012118821032. doi: 10.1177/1536012118821032.

PET Imaging of Hepatocellular Carcinomas: 18F-Fluoropropionic Acid as a Complementary Radiotracer for 18F-Fluorodeoxyglucose.

Zhao J1,2,3, Zhang Z3,4,5, Nie D3,6, Ma H3,4, Yuan G3,4, Su S3,4, Liu S3,4, Liu S1,2, Tang G3,4.

Author information

1
1 Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangzhou, China.
2
2 Department of Nuclear Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
3
3 Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
4
4 Department of Nuclear Medicine and Imaging Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
5
5 Department of Nuclear Medicine, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
6
6 Department of Radiation Oncology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Abstract

OBJECTIVE:

To evaluate the preclinical value of 18F-fluoropropionic acid (18F-FPA) and 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET) for imaging HCCs.

METHODS:

The 18F-FPA and 18F-FDG uptake patterns in 3 HCC cell lines (Hep3B, HepG2, and SK-Hep1) were assessed in vitro and in vivo. The 18F-FPA uptake mechanism was investigated using inhibition experiments with orlistat and 5-tetradecyloxy-2-furoic acid. The 18F-FPA PET imaging was performed in different tumor animal models and compared with 18F-FDG. We also evaluated the expressions of glucose transporter-1 (GLUT1), fatty acid synthase (FASN), and matrix metalloproteinase-2 (MMP2) in these cell lines.

RESULTS:

In vitro experiments showed that the radiotracer uptake patterns were complementary in the HCC cell lines. Orlistat and 5-tetradecyloxy-2-furoic acid decreased the uptake of 18F-FPA. The tumor-to-liver ratio of 18F-FPA was superior to that of 18F-FDG in the SK-Hep1 and HepG2 tumors ( P < .05). However, in the Hep3B tumors, the tumor-to-liver normalized uptake of 18F-FDG was higher than 18F-FPA ( P < .01). FASN was highly expressed in cell lines with high 18F-FPA uptake, whereas GLUT1 was highly expressed in cell lines with high 18F-FDG uptake. The 18F-FPA uptake correlated with FASN ( r = 0.89, P = .014) and MMP2 ( r = 0.77, P = .002) expressions.

CONCLUSIONS:

PET imaging with 18F-FPA combined with 18F-FDG can be an alternative for detecting HCC.

KEYWORDS:

F-fluorodeoxyglucose (F-FDG); F-fluoropropionic acid (F-FPA); hepatocellular carcinoma (HCC); positron emission tomography (PET)

Supplemental Content

Full text links

Icon for Atypon Icon for PubMed Central
Loading ...
Support Center