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Arch Virol. 2019 Apr;164(4):1147-1157. doi: 10.1007/s00705-019-04176-2. Epub 2019 Feb 24.

Porcine epidemic diarrhea virus nsp4 induces pro-inflammatory cytokine and chemokine expression inhibiting viral replication in vitro.

Author information

1
Animal Science College/National Engineering Center for Swine Breeding Industry, South China Agriculture University, No. 483 Wushan Road, Tianhe District, Guangzhou, 510642, China.
2
School of Animal Husbandry and Medical Engineering, Xinyang Agriculture and Forestry University, No. 1 North Road, Pingqiao District, Xinyang, 464000, China.
3
Guangxi State Farms Yongxin Animal Husbandry Group Co., Ltd, No. 135 Qixing Road, Qingxiu District, Nanning, 530000, China.
4
Animal Science College/National Engineering Center for Swine Breeding Industry, South China Agriculture University, No. 483 Wushan Road, Tianhe District, Guangzhou, 510642, China. cxsong2004@163.com.

Abstract

Porcine epidemic diarrhea virus (PEDV) causes severe economic loss in the pig industry each year. To better understand the relationship between cytokines and PEDV replication, in this study, pro-inflammatory cytokine and chemokine expression profiles in Vero cells infected with PEDV were analyzed. Real-time quantitative PCR assay indicated that IL-1α, IL-1β, TNF-α, CCL2, CCL5 and CXCL8 expression levels were significantly upregulated. Moreover, overexpression and siRNA silencing assays showed that overexpression of IL-1α, IL-1β, TNF-α, CCL2, CCL5 and CXCL8 could significantly inhibit PEDV replication, while silencing of IL-1α, IL-1β, TNF-α, CCL2, CCL5 and CXCL8 could significantly promote PEDV replication. Finally, a dual-luciferase reporter assay showed that nsp4 contributed to the expression of IL-1α, IL-1β, TNF-α, CCL2, CCL5 and CXCL8 via the NF-κB pathway. Together, these data determined that PEDV nsp4 could upregulate pro-inflammatory cytokine and chemokine expression, inhibiting viral replication in vitro. These results provided novel insights for understanding the roles of cytokines in PEDV replication.

PMID:
30799511
DOI:
10.1007/s00705-019-04176-2
[Indexed for MEDLINE]

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