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Food Chem Toxicol. 2019 Apr;126:233-247. doi: 10.1016/j.fct.2019.02.017. Epub 2019 Feb 21.

Comparative phytochemical, cytotoxicity, antioxidant and haemostatic studies of Taraxacum officinale root preparations.

Author information

1
Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100, Puławy, Poland.
2
Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Łódź, 90-236, Łódź, Poland.
3
Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Łódź, 90-236, Łódź, Poland. Electronic address: beata.olas@biol.uni.lodz.pl.

Abstract

Although the majority of potentially bioactive components of dandelion root have been described, revealing the presence of hydroxycinnamic acids (HCAs) and sesquiterpene lactones (SLs), new compounds are still being discovered, an example of which are the recently characterized 4-hydroxyphenylacetate inositol esters (PIEs). In this work, the dandelion root was separated into five preparations (A-E) differing in chemical content. A detailed LC-MS and chemical investigation of dandelion fractions allowed the identification of about 100 phytochemicals, including new compounds for the genus Taraxacum, and the plant kingdom, such as amino acid-SL adducts. In the DPPH test, two preparations characterized by high content of HCAs (D and E) showed the highest free radical scavenging activity, while other demonstrated weaker action. In turn, in blood plasma, the best overall protective effect against oxidation by H2O2/Fe was obtained in the presence of preparations A (SL-amino acid adducts enriched fraction) and C (PIEs enriched fraction). A stronger anticoagulant effect was demonstrated for two preparations enriched with HCAs (D and E). None of dandelion root preparations caused the lysis of blood platelets, at all tested range (0.5-50 μg/mL). Our results demonstrate that dandelion roots are a safe and valuable source of different class natural compounds possessing antioxidant, anticoagulant and anti-platelet activities.

KEYWORDS:

Anticoagulant activity; Cytotoxicity; Hemostasis; Oxidative stress; Taraxacum officinale L.; UPLC-PDA-ESI-MS/MS

PMID:
30797873
DOI:
10.1016/j.fct.2019.02.017
[Indexed for MEDLINE]

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