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Int J Biol Macromol. 2019 Jun 1;130:58-67. doi: 10.1016/j.ijbiomac.2019.02.120. Epub 2019 Feb 22.

Preparation and characterisation of a novel silk fibroin/hyaluronic acid/sodium alginate scaffold for skin repair.

Author information

1
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Guangdong Provincial Engineering and Technological Research Center for Drug Carrier Development, Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China.
2
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Guangdong Provincial Engineering and Technological Research Center for Drug Carrier Development, Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China; Beogene Biotech (Guangzhou) Co., Ltd, Guangzhou 510663, China.
3
Beogene Biotech (Guangzhou) Co., Ltd, Guangzhou 510663, China.
4
Guangzhou Chuangseed Biomedical Materials Co., Ltd, Guangzhou 510663, China.
5
The Burns Department of Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
6
The Institute of Biophysics and Cell Engineering of The National Academy of Sciences of Belarus, Minsk 220072, Belarus.
7
Department of Burn and plastic Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China.
8
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Guangdong Provincial Engineering and Technological Research Center for Drug Carrier Development, Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China. Electronic address: guorui@jnu.edu.cn.

Abstract

To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying. Fourier-transform infrared spectroscopy and Raman spectra indicated interactions among SF, HA, and SA molecules. Scanning electron microscopy showed that the prepared SF/HA/SA scaffold had soft, elastic characteristics, with an average pore diameter of 93 μm. Mechanical property, thermogravimetric analyses and degradation results indicated that the SF/HA/SA scaffold had good physical stability in body fluid and mechanical movement-related environments. Cell proliferation, morphological, and live-dead analyses showed that NIH-3T3 fibroblast cells were better able to attach, grow, and proliferate on the SF/HA/SA scaffold compared with SF, SF/HA, and SF/SA scaffolds. We evaluated the wound healing effects in a rat full-thickness burn model. The hematoxylin-eosin (H&E) and Masson's trichrome staining results from SF/HA/SA scaffold showed that improved re-epithelialization, enhanced extracellular matrix remodeling. Our findings showed that the prepared SF/HA/SA scaffold can provide a potential way as a wound dressing for skin repair.

KEYWORDS:

Hyaluronic acid; Scaffold; Silk fibroin; Skin repair; Sodium alginate

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