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J Glob Antimicrob Resist. 2019 Mar;16:251-253. doi: 10.1016/j.jgar.2019.02.005. Epub 2019 Feb 20.

Draft genome sequences of three clinical isolates of teicoplanin-resistant Staphylococcus epidermidis from patients without prior exposure to glycopeptide antibiotics.

Author information

1
Laboratory for Biology of Secondary Metabolism, Institute of Microbiology of the Academy of Sciences of the Czech Republic, BIOCEV, Průmyslová 595, 252 50 Vestec, Czech Republic. Electronic address: vladimir.vimberg@biomed.cas.cz.
2
Laboratory for Biology of Secondary Metabolism, Institute of Microbiology of the Academy of Sciences of the Czech Republic, BIOCEV, Průmyslová 595, 252 50 Vestec, Czech Republic.
3
Clinical Microbiology and ATB Centre, Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital, Prague, Czech Republic.
4
Clinical Microbiology and ATB Centre, Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital, Prague, Czech Republic; Department of Medical Microbiology, Medical Faculty of Palacký University, Olomouc, Czech Republic.

Abstract

OBJECTIVES:

The aim of this study was to analyse the DNA sequences of three teicoplanin-resistant Staphylococcus epidermidis isolates collected from patients not previously treated with glycopeptide antibiotics.

METHODS:

The minimum inhibitory concentrations (MICs) of 12 antibiotics, including teicoplanin and vancomycin, were determined by the broth microdilution method. Genomic DNA was isolated, was sequenced by HiSeqX paired-end sequencing and was assembled into draft genome sequences using MyPro pipeline.

RESULTS:

Analysis of the draft genome sequences demonstrated that the teicoplanin-resistant S. epidermidis isolates belonged to multilocus sequence typing (MLST) sequence types ST5 and ST87 and encoded multiple antimicrobial resistance genes, including the methicillin resistance gene mecA.

CONCLUSIONS:

This report highlights the risk of dissemination of S. epidermidis strains resistant to a wide range of clinically important antibiotics.

KEYWORDS:

Staphylococcus epidermidis; Teicoplanin resistance; Whole-genome sequencing

PMID:
30797086
DOI:
10.1016/j.jgar.2019.02.005

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