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Eur J Pharmacol. 2019 May 15;851:151-160. doi: 10.1016/j.ejphar.2019.02.033. Epub 2019 Feb 21.

Diabetes-induced damage of gastric nitric oxide neurons mediated by P2X7R in diabetic mice.

Author information

1
Department of Pediatric Surgery, Xin Hua Hospital, Affiliated to Shanghai JiaoTong University School of Medicine, 1665 Kong Jiang Road, 200092 Shanghai, India; Department of Physiology, Shanghai Jiaotong University School of Medicine, Shanghai 200240, China.
2
Department of Physiology, Shanghai Jiaotong University School of Medicine, Shanghai 200240, China.
3
Department of Physiology, Chungbuk National University College of Medicine, Cheongju, Chungbuk 361-763, Republic of Korea.
4
Department of Pediatric Surgery, Xin Hua Hospital, Affiliated to Shanghai JiaoTong University School of Medicine, 1665 Kong Jiang Road, 200092 Shanghai, India. Electronic address: jiechen1974@163.com.
5
Department of Physiology, Shanghai Jiaotong University School of Medicine, Shanghai 200240, China. Electronic address: wenxiexu@sjtu.edu.cn.

Abstract

It is generally considered that enteric neuropathy is one of the causative factors in diabetic gastroparesis. Our previous study demonstrated that there is a loss of NOS neurons in diabetic mice. However, the underlying mechanism remains unclear. The present study was designed to clarify the relationship between neuronal P2X7R and NOS neuron damage. The effect of P2X7R on diabetes-induced gastric NOS neurons damage and its mechanism were investigated by using quantitative RT-PCR´╝îimmunofluorescence, western blot, isometric force recording, intracellular calcium ([Ca2+]i) measurement and whole-cell patch clamp techniques. The immunohistochemistry and western blot results showed that nNOS expression was significantly down-regulated in diabetic mice, meanwhile, electric field stimulation-induced NOS sensitive relaxation was significantly suppressed. Myenteric neurons expressed P2X7R and pannexin1, and the mRNA and protein level of P2X7R and pannexin1 were up-regulated in diabetic mice. BzATP, a P2X7R activator, evoked [Ca2+]i increase in Hek293 cells with heterologous expression of P2X7R (Hek293-P2X7R cells) and the same dose of ATP-induced [Ca2+]i was more obvious in Hek293-P2X7R cells than in Hek293 cells. Application of BzATP activated an inward current of Hek293-P2X7R in a dose dependent manner. Hek293-P2X7R but not untransfected Hek293 cells could take up of YO-PRO-1. In addition, the uptake of YO-PRO-1 by Hek293-P2X7R was blocked by oxATP, a P2X7 antagonist and CBX, a pannexin1 inhibitor. The results suggest that the P2X7R of enteric neurons may be involved in diabetes-induced NOS neuron damage via combining with pannexin-1 to form transmembrane pores which induce macromolecular substances and calcium into the cells.

KEYWORDS:

Diabetic mellitus; NOS neurons; P2X7R; Pannexin1; Transmembrane pore

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