Format

Send to

Choose Destination
Cell Mol Immunol. 2019 Mar;16(3):242-249. doi: 10.1038/s41423-019-0214-4. Epub 2019 Feb 22.

Revolutionizing immunology with single-cell RNA sequencing.

Chen H1,2,3, Ye F1, Guo G4,5,6,7,8.

Author information

1
Center for Stem Cell and Regenerative Medicine, Zhejiang University School of Medicine, 310058, Hangzhou, China.
2
Bone Marrow Transplantation Center, The First Affiliated Hospital, Zhejiang University School of Medicine, 310009, Hangzhou, China.
3
Zhejiang Provincial Key Lab for Tissue Engineering and Regenerative Medicine, Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, 310058, Hangzhou, China.
4
Center for Stem Cell and Regenerative Medicine, Zhejiang University School of Medicine, 310058, Hangzhou, China. ggj@zju.edu.cn.
5
Bone Marrow Transplantation Center, The First Affiliated Hospital, Zhejiang University School of Medicine, 310009, Hangzhou, China. ggj@zju.edu.cn.
6
Zhejiang Provincial Key Lab for Tissue Engineering and Regenerative Medicine, Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, 310058, Hangzhou, China. ggj@zju.edu.cn.
7
Institute of Hematology, Zhejiang University, 310058, Hangzhou, China. ggj@zju.edu.cn.
8
Stem Cell Institute, Zhejiang University, 310058, Hangzhou, China. ggj@zju.edu.cn.

Abstract

The immune system is composed of a complex hierarchy of cell types that protect the organism against disease and maintain homeostasis. Identifying heterogeneity of immune cells is the key to understanding the immune system. Advanced single-cell RNA sequencing (scRNA-seq) technologies are revolutionizing our ability to study immunology. By measuring transcriptomes at the single-cell level, scRNA-seq enables identification of cellular heterogeneity in far greater detail than conventional methods. In this review, we introduce the existing scRNA-seq technologies and present their strengths and weaknesses. We also discuss potential applications and future innovations of scRNA-seq in immunology.

PMID:
30796351
DOI:
10.1038/s41423-019-0214-4

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center