Format

Send to

Choose Destination
Micromachines (Basel). 2019 Feb 21;10(2). pii: E143. doi: 10.3390/mi10020143.

The Effect of Microfluidic Geometry on Myoblast Migration.

Author information

1
Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA. rxa162330@utdallas.edu.
2
Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA. bjb140530@utdallas.edu.
3
Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA. Kevin.Lam2@utdallas.edu.
4
Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA. vms150130@utdallas.edu.
5
Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA. Joseph.Pancrazio@utdallas.edu.
6
Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA. David.Schmidtke@utdallas.edu.
7
Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA. Nesreen.Alsmadi@utdallas.edu.

Abstract

In vitro systems comprised of wells interconnected by microchannels have emerged as a platform for the study of cell migration or multicellular models. In the present study, we systematically evaluated the effect of microchannel width on spontaneous myoblast migration across these microchannels-from the proximal to the distal chamber. Myoblast migration was examined in microfluidic devices with varying microchannel widths of 1.5⁻20 µm, and in chips with uniform microchannel widths over time spans that are relevant for myoblast-to-myofiber differentiation in vitro. We found that the likelihood of spontaneous myoblast migration was microchannel width dependent and that a width of 3 µm was necessary to limit spontaneous migration below 5% of cells in the seeded well after 48 h. These results inform the future design of Polydimethylsiloxane (PDMS) microchannel-based co-culture platforms as well as future in vitro studies of myoblast migration.

KEYWORDS:

PDMS; microfabrication; microfluidics; migration; myoblasts

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center