Format

Send to

Choose Destination
Nanomedicine. 2019 Apr;17:266-275. doi: 10.1016/j.nano.2019.02.002. Epub 2019 Feb 20.

Nanoparticle physicochemical properties determine the activation of intracellular complement.

Author information

1
Nanotechnology Characterization Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD.
2
Department of Chemistry & Biochemistry, Texas Christian University, Fort Worth, TX.
3
Protein Characterization Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, sponsored by the National Cancer Institute, Frederick, MD.
4
Antibody Characterization Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD.
5
Nanotechnology Characterization Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD. Electronic address: marina@mail.nih.gov.

Abstract

The complement system plays an essential role in both innate and adaptive immunity. The traditional understanding of this system comes from studies investigating complement proteins produced by the liver and present in plasma to "complement" the immune cell-mediated response to invading pathogens. Recently, it has been reported that immune cells including, but not limited to, T-cells and monocytes, express complement proteins. This complement is referred to as intracellular (IC) and implicated in the regulation of T-cell activation. The mechanisms and the structure-activity relationship between nanomaterials and IC, however, are currently unknown. Herein, we describe a structure-activity relationship study demonstrating that under in vitro conditions, only polymeric materials with cationic surfaces activate IC in T-cells. The effect also depends on particle size and occurs through a mechanism involving membrane damage, thereby IC on the cell surface serves as a self-opsonization marker in response to the nanoparticle-triggered danger affecting the cell integrity.

KEYWORDS:

Anaphylatoxins; CARPA; Complement; Hypersensitivity; IC; In vitro; Infusion reactions; Nanoparticles; Preclinical; T-cells

PMID:
30794962
PMCID:
PMC6520145
[Available on 2020-04-01]
DOI:
10.1016/j.nano.2019.02.002

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center