HEWL interacts with dissipated oleic acid micelles, and decreases oleic acid cytotoxicity

PLoS One. 2019 Feb 22;14(2):e0212648. doi: 10.1371/journal.pone.0212648. eCollection 2019.

Abstract

Senile plaques are well-known hallmarks of Alzheimer's Diseases (AD). However, drugs targeting tangles of the protein tau and plaques of β-amyloid have no significant effect on disease progression, and the studies on the underlying mechanism of AD remain in high demand. Growing evidence supports the protective role of senile plaques in local inflammation driven by S100A9. We herein demonstrate that oleic acid (OA) micelles interact with hen egg white lysozyme (HEWL) and promote its amyloid formation. Consequently, SH-SY5Y cell line and mouse neural stem cells are rescued from OA toxicity by co-aggregation of OA and HEWL. Using atomic force microscopy in combination with fluorescence microscopy, we revealed that HEWL forms round-shaped aggregates in the presence of OA micelles instead of protofibrils of HEWL alone. These HEWL amyloids act as a sink for toxic OA micelles and their co-aggregate form large clumps, suggesting a protective function in amyloid and OA cytotoxicity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / metabolism
  • Amyloid / chemistry*
  • Animals
  • Cell Line
  • Chickens
  • Humans
  • Micelles*
  • Muramidase / chemistry*
  • Muramidase / metabolism
  • Oleic Acid / chemistry*
  • Oleic Acid / metabolism
  • Protein Aggregation, Pathological / metabolism
  • Rats

Substances

  • Amyloid
  • Micelles
  • Oleic Acid
  • hen egg lysozyme
  • Muramidase

Grants and funding

This work was supported by the Chongqing Basic and Frontier Research Program. Project (cstc2015jcyjA10034), Swedish Medical Research Council (2014-3241 to L.A.M.-R.), Insamlingsstiftelsen (FS 2.1.12-1605-14 to L.A.M.-R.), Biochemical Imaging Platform, Umeå University (to L.A.M.-R.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.