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Int J Exp Pathol. 2018 Dec;99(6):304-311. doi: 10.1111/iep.12302.

Nicotinamide riboside has protective effects in a rat model of mesenteric ischaemia-reperfusion.

Author information

1
V.A. Almazov National North-West Medical Research Center, Ministry of Health of the Russian Federation, Saint-Petersburg, Russia.
2
Saint-Petersburg State Chemical Pharmaceutical University, Saint-Petersburg, Russia.
3
Zoological Institute, Russian Academy of Sciences, Universitetskaya Embankment 1Saint Petersburg, 199034, Russia.
4
Saint-Petersburg National Research University of Information Technologies, Mechanics and Optics, Saint-Petersburg, Russia.
5
Pavlov First Saint Petersburg State Medical University, Ministry of Health of the Russian Federation, Saint-Petersburg, Russia.
6
Research Institute of Influenza Ministry of Health of the Russian Federation, Saint-Petersburg, Russia.
7
Children's Scientific and Clinical Center of Infectious Diseases Federal Medical and Biological Agency, Saint-Petersburg, Russia.

Abstract

Acute mesenteric ischaemia is a syndrome caused by inadequate blood flow through the mesenteric vessels, resulting in ischaemia and eventual gangrene of the bowel wall. Although relatively rare, it is a potentially life-threatening condition. The maintenance of haemodynamic stability, along with adequate oxygen saturation, and the correction of any electrolyte imbalance, are of the utmost importance. However, nicotinamide adenine dinucleotide (NAD) biosynthesis modulation by precursor introduction can also be a powerful tool for preventing injury. Nicotinamide riboside is a pyridine-nucleoside form of vitamin B3 that functions as a precursor to NAD+ . The present study investigated nicotinamide riboside's effect on endothelium functional state, microcirculation and intestinal morphology in acute mesenteric ischaemia and reperfusion. Mesenteric ischaemia was simulated after the adaptation period (15 minutes) by occluding the superior mesenteric artery for 60 minutes, followed by a reperfusion period of 30 minutes. The functional state of intestinal microcirculation was evaluated by laser Doppler flowmetry. Endothelial functional activity was studied by using wire myography. Intestinal samples were stained with haematoxylin and eosin for histological analysis. The results revealed that nicotinamide riboside protects the intestinal wall from ischaemia-reperfusion injury, as well as improving the relaxation function of mesenteric vessels. Nicotinamide riboside's protective effect in small intestine ischaemia-reperfusion can be used to reduce ischaemia-reperfusion injury, as well as to preserve intestinal grafts until transplant.

KEYWORDS:

acute mesenteric ischaemia; ischaemia-reperfusion injury; microcirculation; nicotinamide riboside; wire myography

PMID:
30793416
PMCID:
PMC6384503
[Available on 2019-12-01]
DOI:
10.1111/iep.12302
[Indexed for MEDLINE]

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