Format

Send to

Choose Destination
Sci Rep. 2019 Feb 21;9(1):2485. doi: 10.1038/s41598-018-37697-y.

Comparative transcriptome analysis reveals differentially expressed genes related to the tissue-specific accumulation of anthocyanins in pericarp and aleurone layer for maize.

Author information

1
Corn Research Center, Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, P. R. China.
2
School of Life Sciences, Anhui Agricultural University, Hefei, 230036, P. R. China.
3
School of Life Sciences, Anhui Agricultural University, Hefei, 230036, P. R. China. hhfan0551@126.com.
4
School of Life Sciences, Anhui Agricultural University, Hefei, 230036, P. R. China. xinzhang@ahau.edu.cn.
5
Corn Research Center, Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, P. R. China. ybzhou99@126.com.

Abstract

Purple corn is a rich source of anthocyanins. In the experiment, two anthocyanins-enriched purple corn lines Ha0414 and Ha6130 were identified. The anthocyanins were respectively accumulated in the pericarp of Ha0414 and the aleurone layer of Ha6130 with different composition and content. Transcriptome analysis of the two tissues in both lines identified 16 and 14 differentially expressed genes belonging to anthocyanin metabolism pathway in pericarp and the aleurone layer, individually. Of these genes, two genes encoding 2-oxoglutarate (2OG) and Fe (II)-dependent oxygenase superfamily proteins, and one gene annotated as UDP-glycosyltransferase superfamily protein exhibited increased transcript abundance in both the colored pericarp and aleurone layer. Otherwise, one gene annotated as flavonoid 3', 5'-hydroxylase, and another gene encoding flavonoid 3'-monooxygenase displayed increased transcript abundance in the aleurone layer of Ha6130. Moreover, 36 transcription factors were identified with increased transcript abundance in the pericarp of Ha0414, such as bHLH transcription factors, WRKY transcription factors, and HB transcription factors. And 79 transcription factors were isolated with an increased expression level in the aleurone layer of Ha6130, including MYB transcription factors, MYB-related transcription factors, and bHLH transcription factors. These genes expression may result in the tissue-specific accumulation of anthocyanins in pericarp and aleurone layer.

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center