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Cancer Lett. 2019 May 1;449:215-225. doi: 10.1016/j.canlet.2019.02.031. Epub 2019 Feb 18.

BRM transcriptionally regulates miR-302a-3p to target SOCS5/STAT3 signaling axis to potentiate pancreatic cancer metastasis.

Author information

1
Department of General Surgery, Peking University First Hospital, Beijing, China.
2
Department of Endoscopy Center, Peking University First Hospital, Beijing, China.
3
Department of Surgical Oncology, Peking University Ninth School of Clinical Medicine (Beijing Shijitan Hospital, Capital Medical University), Beijing, China.
4
Clinic of General, Visceral and Transplantation Surgery, University of Ulm, Ulm, Germany.
5
Department of General Surgery, Peking University First Hospital, Beijing, China. Electronic address: tianxiaodong@pkufh.cn.
6
Department of General Surgery, Peking University First Hospital, Beijing, China. Electronic address: yangyinmosci@163.com.

Abstract

Brahma (BRM) has recently been documented as a significant predictor of pancreatic cancer (PC) metastasis. This study aimed to further elucidate molecular mechanism by which BRM promotes PC metastasis. We found that silencing BRM reduced PC cell migration and invasion both in vivo and in vitro, accompanied by reduced level of miR-302a-3p. BRM positively regulated the transcription of miR-302a-3p, which acted as a metastasis-promoting miRNA in PC cells. miR-302a-3p directly targeted SOCS5 to boost STAT3 phosphorylation and induce the transcription of STAT3 target genes. Furthermore, miR-302a-3p level was higher in tissue and plasma samples derived from PC patients, and was significantly associated with worse clinical pathological features. In xenograft models, inhibiting miR-302a-3p was synergistically lethal in BRM-silenced PC cells. In conclusion, our results suggest that transcriptional regulation of miR-302a-3p by BRM potentiates PC metastasis by epigenetically suppressing SOCS5 expression and activating STAT3 signaling. These new findings provide potential therapeutic avenues for preventing PC-associated death.

KEYWORDS:

BRM; Metastasis; Pancreatic cancer; SOCS5; STAT3; miR-302a-3p

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