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PLoS Biol. 2019 Feb 21;17(2):e3000152. doi: 10.1371/journal.pbio.3000152. eCollection 2019 Feb.

Single-cell transcriptomics reveals gene expression dynamics of human fetal kidney development.

Author information

1
Leiden Institute of Physics, Leiden University, Leiden, The Netherlands.
2
Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.
3
Department of Reproductive Medicine, Ghent University Hospital, Ghent, Belgium.

Abstract

The current understanding of mammalian kidney development is largely based on mouse models. Recent landmark studies revealed pervasive differences in renal embryogenesis between mouse and human. The scarcity of detailed gene expression data in humans therefore hampers a thorough understanding of human kidney development and the possible developmental origin of kidney diseases. In this paper, we present a single-cell transcriptomics study of the human fetal kidney. We identified 22 cell types and a host of marker genes. Comparison of samples from different developmental ages revealed continuous gene expression changes in podocytes. To demonstrate the usefulness of our data set, we explored the heterogeneity of the nephrogenic niche, localized podocyte precursors, and confirmed disease-associated marker genes. With close to 18,000 renal cells from five different developmental ages, this study provides a rich resource for the elucidation of human kidney development, easily accessible through an interactive web application.

Conflict of interest statement

The authors have declared that no competing interests exist.

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