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Int J Syst Evol Microbiol. 2019 Feb 21. doi: 10.1099/ijsem.0.003306. [Epub ahead of print]

Characterization of the phylogenetic diversity of five novel species belonging to the genus Bifidobacterium: Bifidobacterium castoris sp. nov., Bifidobacterium callimiconis sp. nov., Bifidobacterium goeldii sp. nov., Bifidobacterium samirii sp. nov. and Bifidobacterium dolichotidis sp. nov.

Author information

1
1​Laboratory of Probiogenomics, Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy.
2
2​Department of Veterinary Medical Science, University of Parma, Parma, Italy.
3
3​APC Microbiome Institute and School of Microbiology, National University of Ireland, Cork, Ireland.

Abstract

Five Bifidobacterium strains, i.e. 2020BT, 2028BT, 2033BT, 2034BT and 2036BT, were isolated from European beaver (Castor fiber), Goeldi's marmoset (Callimicogoeldii), black-capped squirrel monkey (Saimiriboliviensissubsp. peruviensis) and Patagonian mara (Dolichotispatagonum). All of these isolates were shown to be Gram-positive, facultative anaerobic, d-fructose 6-phosphate phosphoketolase-positive, non-motile and non-sporulating. Phylogenetic analyses based on 16S rRNA gene sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2020BT, 2028BT, 2033BT, 2034BT and 2036BT exhibit close phylogenetic relatedness to Bifidobacteriumbiavatii DSM 23969T, Bifidobacteriumbifidum LMG 11041T, Bifidobacteriumchoerinum LMG 10510T, Bifidobacteriumgallicum LMG 11596T, Bifidobacteriumimperatoris LMG 30297T, Bifidobacteriumitalicum LMG 30187T and Bifidobacteriumvansinderenii LMG 30126T, respectively. Further genotyping based on the genome sequence of the isolated strains combined with phenotypic analyses, clearly show that these strains are distinct from each of the type strains of the so far recognized Bifidobacterium species. Thus, Bifidobacteriumcastoris sp. nov. (2020BT=LMG 30937T=CCUG 72816T), Bifidobacteriumcallimiconis sp. nov. (2028BT=LMG 30938T=CCUG 72814T), Bifidobacteriumsamirii sp. nov. (2033BT=LMG 30940T=CCUG 72817T), Bifidobacteriumgoeldii sp. nov. (2034BT=LMG 30939T=CCUG 72815T) and Bifidobacteriumdolichotidis sp. nov. (2036BT=LMG 30941T=CCUG 72818T) are proposed as novel Bifidobacterium species.

PMID:
30789326
DOI:
10.1099/ijsem.0.003306

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