Mouse Hematopoietic Stem Cell Modification and Labelling by Transduction and Tracking Posttransplantation

Benjamin Cao; Songhui Li; Claire Pritchard; Brenda Williams; Susan K Nilsson

doi:10.1007/978-1-4939-9086-3_9

Mouse Hematopoietic Stem Cell Modification and Labelling by Transduction and Tracking Posttransplantation

Methods Mol Biol. 2019:1940:129-142. doi: 10.1007/978-1-4939-9086-3_9.

Authors

Benjamin Cao^{1

2}, Songhui Li^{1

2}, Claire Pritchard^{1

2}, Brenda Williams^{1

2}, Susan K Nilsson^{3

4}

Affiliations

¹ Biomedical Manufacturing, CSIRO Clayton, Clayton, VIC, Australia.
² Australian Regenerative Medicine Institute, Monash University, Clayton, VIC, Australia.
³ Biomedical Manufacturing, CSIRO Clayton, Clayton, VIC, Australia. susie.nilsson@csiro.au.
⁴ Australian Regenerative Medicine Institute, Monash University, Clayton, VIC, Australia. susie.nilsson@csiro.au.

PMID: 30788822
DOI: 10.1007/978-1-4939-9086-3_9

Abstract

The tracking of the hematopoietic potential of genetically manipulated fluorescent hematopoietic stem cells (HSC) in the bone marrow (BM) allows the assessment of regulatory processes involved in the re-establishment of hematopoiesis posttransplant. Herein, we describe the means to assess the consequence of expressing specific genes in HSC on their engraftment potential posttransplant.

Keywords: Bone marrow transplantation; Hematopoietic stem cells; Lentiviral transduction.

MeSH terms

Animals
Bone Marrow / physiology
Bone Marrow Cells / cytology*
Cell Movement / physiology
Gene Expression
Hematopoiesis / physiology*
Hematopoietic Stem Cell Transplantation*
Hematopoietic Stem Cells / cytology*
Lentivirus / genetics
Mice
Mice, Inbred C57BL
Staining and Labeling / methods
Stem Cell Niche / physiology*
Transduction, Genetic