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Methods Mol Biol. 2019;1927:1-9. doi: 10.1007/978-1-4939-9142-6_1.

Pathway and Gene Discovery from Natural Hosts and Organisms.

Author information

1
U.S. Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Walnut Creek, CA, USA.
2
U.S. Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Walnut Creek, CA, USA. yyoshikuni@lbl.gov.

Abstract

Information in public sequence databases on the genomes and metagenomes of microbes and plants has grown rapidly. In conjunction with technological developments in computational identification of biosynthetic gene clusters, molecular biology, synthetic biology, and analytical tools, this has revealed genes for enzymes with optimal and targeted function, as well as a rich pool of uncharacterized metabolic pathways. This chapter discusses different approaches to discovery of genes and metabolic pathways in microbes and plants in nature, such as genomic mining, transcriptome analysis, and metabolite profiling.

KEYWORDS:

Computational identification; Enzyme; Gene cluster; Gene library; Gene sequence; Metabolome; Metagenomics; Pathway; Transcriptome

PMID:
30788781
DOI:
10.1007/978-1-4939-9142-6_1
[Indexed for MEDLINE]

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