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Am J Clin Pathol. 2019 Feb 21. pii: aqz003. doi: 10.1093/ajcp/aqz003. [Epub ahead of print]

The Utility of Phosphohistone H3 (PHH3) in Follicular Lymphoma Grading.

Author information

1
Department of Pathology, Brooke Army Medical Center, San Antonio, TX.

Abstract

OBJECTIVES:

To determine the utility of phosphohistone H3 (PHH3) mitotic count (MC) in grading follicular lymphoma (FL).

METHODS:

FL cases were identified (2005-2017). Three hematopathologists recorded their average Ki-67 proliferation index, MC/high-power field (hpf) using PHH3 and H&E stains, and number of centroblasts/hpf. Results were assessed for correlations and interobserver variability.

RESULTS:

Forty-three cases of FL were studied. PHH3 MC resulted in the strongest correlation to grade (r = 0.701, P < .0001) compared with Ki-67 proliferation index (PI) (r = 0.681, P < .0001) and H&E MC (r = 0.536, P = .0002) and the strongest linear relationship to centroblast count (R2 = 0.453). Agreement among pathologists was strongest for PHH3 (intraclass correlation coefficient [ICC] = 0.86) followed by Ki-67 PI (ICC = 0.85) and H&E MC (ICC = 0.78).

CONCLUSIONS:

PHH3 correlates best to histologic grade and has less interobserver variability compared with Ki-67 PI and H&E MC. These results support using PHH3 as an adjunct in FL grading.

KEYWORDS:

Follicular; Grading; Lymphoma; PHH3; Phosphohistone H3

PMID:
30788495
DOI:
10.1093/ajcp/aqz003

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