Format

Send to

Choose Destination
Nutr Res Pract. 2019 Feb;13(1):3-10. doi: 10.4162/nrp.2019.13.1.3. Epub 2018 Dec 29.

Nicotinamide riboside regulates inflammation and mitochondrial markers in AML12 hepatocytes.

Author information

1
Department of Food and Nutrition, Seoul Women's University, 621 Hwarangro, Nowon-Gu, Seoul 01797, Korea.

Abstract

BACKGROUND/OBJECTIVES:

The NAD+ precursor nicotinamide riboside (NR) is a type of vitamin B3 found in cow's milk and yeast-containing food products such as beer. Recent studies suggested that NR prevents hearing loss, high-fat diet-induced obesity, Alzheimer's disease, and mitochondrial myopathy. The objective of this study was to investigate the effects of NR on inflammation and mitochondrial biogenesis in AML12 mouse hepatocytes.

MATERIALS/METHODS:

A subset of hepatocytes was treated with palmitic acid (PA; 250 µM) for 48 h to induce hepatocyte steatosis. The hepatocytes were treated with NR (10 µM and 10 mM) for 24 h with and without PA. The cell viability and the levels of sirtuins, inflammatory markers, and mitochondrial markers were analyzed.

RESULTS:

Cytotoxicity of NR was examined by PrestoBlue assay. Exposure to NR had no effect on cell viability or morphology. Gene expression of sirtuin 1 (Sirt1) and Sirt3 was significantly upregulated by NR in PA-treated hepatocytes. However, Sirt1 activities were increased in hepatocytes treated with low-dose NR. Hepatic pro-inflammatory markers including tumor necrosis factor-alpha and interleukin-6 were decreased in NR-treated cells. NR upregulated anti-inflammatory molecule adiponectin, and, tended to down-regulate hepatokine fetuin-A in PA-treated hepatocytes, suggesting its inverse regulation on these cytokines. NR increased levels of mitochondrial markers including peroxisome proliferator-activated receptor γ coactivator-1α, carnitine palmitoyltransferase 1, uncoupling protein 2, transcription factor A, mitochondrial and mitochondrial DNA in PA-treated hepatocytes.

CONCLUSIONS:

These data demonstrated that NR attenuated hepatic inflammation and increased levels of mitochondrial markers in hepatocytes.

KEYWORDS:

Fatty liver; inflammation; mitochondria; niacin

Supplemental Content

Full text links

Icon for The Korean Nutrition Society; The Korean Society of Community Nutrition Icon for PubMed Central
Loading ...
Support Center