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Front Microbiol. 2019 Feb 6;10:179. doi: 10.3389/fmicb.2019.00179. eCollection 2019.

Cross-Border Transmission of Salmonella Choleraesuis var. Kunzendorf in European Pigs and Wild Boar: Infection, Genetics, and Evolution.

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European Union Reference Laboratory for Antimicrobial Resistance, WHO Collaborating Center for Antimicrobial Resistance in Food Borne Pathogens and Genomics, Research Group for Genomic Epidemiology, National Food Institute, Kongens Lyngby, Denmark.
RISE Research Institutes of Sweden, Lund, Sweden.
Istituto Zooprofilattico Sperimentale del Lazio e della Toscana, National Reference Laboratory for Antimicrobial Resistance, Rome, Italy.
National Salmonella Reference Laboratory, Unit Molecular Microbiology and Genome Analysis, Federal Institute for Risk Assessment, Berlin, Germany.
National Veterinary Research Institute Department of Microbiology, National Reference Laboratory for Salmonellosis and Antimicrobial Resistance, Puławy, Poland.
Department of Agriculture, Food and the Marine Laboratories, Celbridge, Ireland.
Division of Animal and Food Microbiology, Center for Veterinary Medicine, United States Food and Drug Administration, Laurel, MD, United States.
French Agency for Food, Environmental and Occupational Health and Safety, Maisons-Alfort, France.
NRC Salmonella, Austrian Agency for Health and Food Safety, Graz, Austria.
Veterinaar- ja Toidulaboratoorium, Tartu, Estonia.
Bakteriológiai Laboratórium, Állategészségügyi Diagnosztikai Igazgatóság, Nemzeti Élelmiszerlánc-biztonsági Hivatal, Budapest, Hungary.
Státní Veterinární Ústav Praha, Prague, Czechia.
National Veterinary Institute, Technical University of Denmark, Kongens Lyngby, Denmark.


Salmonella enterica subspecies enterica serotype Choleraesuis is a swine adapted serovar. S. Choleraesuis variant Kunzendorf is responsible for the majority of outbreaks among pigs. S. Choleraesuis is rare in Europe, although there have been serious outbreaks in pigs including two outbreaks in Denmark in 1999-2000 and 2012-2013. Here, we elucidate the epidemiology, possible transmission routes and sources, and clonality of European S. Choleraesuis isolates including the Danish outbreak isolates. A total of 102 S. Choleraesuis isolates from different European countries and the United States, covering available isolates from the last two decades were selected for whole genome sequencing. We applied a temporally structured sequence analysis within a Bayesian framework to reconstruct a temporal and spatial phylogenetic tree. MLST type, resistance genes, plasmid replicons, and accessory genes were identified using bioinformatics tools. Fifty-eight isolates including 11 out of 12 strains from wild boars were pan-susceptible. The remaining isolates carried multiple resistance genes. Eleven different plasmid replicons in eight plasmids were determined among the isolates. Accessory genes were associated to the identified resistance genes and plasmids. The European S. Choleraesuis was estimated to have emerged in ∼1837 (95% credible interval, 1733-1983) with the mutation rate of 1.02 SNPs/genome/year. The isolates were clustered according to countries and neighbor countries. There were transmission events between strains from the United States and European countries. Wild boar and pig isolates were genetically linked suggesting cross-border transmission and transmission due to a wildlife reservoir. The phylogenetic tree shows that multiple introductions were responsible for the outbreak of 2012-2013 in Denmark, and suggests that poorly disinfected vehicles crossing the border into Denmark were potentially the source of the outbreak. Low levels of single nucleotide polymorphisms (SNPs) differences (0-4 SNPs) can be observed between clonal strains isolated from different organs of the same animal. Proper disinfection of livestock vehicles and improved quality control of livestock feed could help to prevent future spread of S. Choleraesuis or other more serious infectious diseases such as African swine fever (ASF) in the European pig production system.


Kunzendorf; Salmonella Choleraesuis; antimicrobial resistance genes; epidemiology; phylogenetics; transmission; whole genome sequencing; wild boar

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