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Biofouling. 2019 Jan;35(1):50-58. doi: 10.1080/08927014.2018.1563892. Epub 2019 Feb 21.

Colonization and growth of dehalorespiring biofilms on carbonaceous sorptive amendments.

Author information

1
a Department of Civil and Environmental Engineering , University of Maryland , College Park , MD , USA.
2
b Geosyntec Consultants , Columbia , MD , USA.
3
c Department of Civil Engineering , Universidad de Chile , Santiago , Chile.
4
d Department of Marine Biotechnology, Institute of Marine and Environmental Technology , University of Maryland Baltimore County , Baltimore , MD , USA.

Abstract

Removal of polychlorinated biphenyls (PCBs) from contaminated sediments is a priority due to accumulation in the food chain. Recent success with reduction of PCB bioavailability due to adsorption onto activated carbon led to the recognition of in situ treatment as a remediation approach. In this study, reduced bioavailability and subsequent break-down of PCBs in dehalorespiring biofilms was investigated using Dehalobium chlorocoercia DF1. DF1 formed a patchy biofilm ranging in thickness from 3.9 to 6.7 µm (average 4.6 ± 0.87 µm), while the biofilm coverage varied from 5.5% (sand) to 20.2% (activated carbon), indicating a preference for sorptive materials. Quantification of DF1 biofilm bacteria showed 1.2-15.3 × 109 bacteria per gram of material. After 22 days, coal activated carbon, bone biochar, polyoxymethylene, and sand microcosms had dechlorinated 73%, 93%, 100%, and 83%, respectively. These results show that a biofilm-based inoculum for bioaugmentation of PCBs in sediment can be an efficient approach.

KEYWORDS:

Dehalorespiring biofilms; activated carbon; contaminated sediment; polychlorinated biphenyls (PCBs); quantitative confocal laser scanning microscopy (Q-CLSM)

PMID:
30786761
DOI:
10.1080/08927014.2018.1563892
[Indexed for MEDLINE]

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