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Rev Inst Med Trop Sao Paulo. 2019 Feb 14;61:e8. doi: 10.1590/S1678-9946201961008.

Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients.

Author information

1
Fundação Oswaldo Cruz, Instituto Nacional de Infectologia Evandro Chagas, Laboratório de Micologia, Setor Imunodiagnóstico, Rio de Janeiro, Rio de Janeiro, Brazil.
2
Hospital São José de Doenças Infecciosas, Fortaleza, Ceará, Brazil.
3
Universidad Nacional Autónoma de México, Facultad de Medicina, Departamento de Microbiología y Parasitología, Laboratorio de Inmunología de Hongos, Ciudad de México, México.
4
Universidade Federal do Ceará, Faculdade de Medicina, Departamento de Saúde Comunitária, Fortaleza, Ceará, Brazil.

Abstract

Mixed infection by Histoplasma capsulatum isolates with different mating types, in AIDS-patients are described in this study. Morphological, mating type-specific PCR assay and multilocus sequencing type analysis of H. capsulatum isolates recovered from two Brazilian AIDS-patients were performed. Five H. capsulatum isolates were recovered at different times from the two patients. Three isolates were obtained from bone marrow (day 1 - CE0411) and buffy coat cultures (day 1 - CE0311; day 2 - CE0511) of patient 1, and two isolates were isolated from buffy coat cultures (day 3 - CE2813; day 12 - CE2513) of patient 2. The mycelial colonies depicted different textures and pigmentation features. Dimorphic conversion to the yeast-phase in ML-Gema medium was achieved in all isolates. MAT1-1 idiomorph was identified in CE0311, CE0411 and CE2813 isolates; MAT1-2 idiomorph was found in CE0511 and CE2513 isolates. These H. capsulatum isolates were grouped within LAm A clade, highlighting that CE0311 and CE0411 isolates formed a subgroup supported by a high bootstrap value. The CE0511, CE2513, and CE2813 isolates clustered together with a Brazilian H151 isolate. This research reports mixed infections caused by H. capsulatum isolates with different mating types in Brazilian AIDS-patients for the first time in the literature.

PMID:
30785562
PMCID:
PMC6376931
DOI:
10.1590/S1678-9946201961008
[Indexed for MEDLINE]
Free PMC Article

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