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Indian J Dermatol Venereol Leprol. 2019 Feb 20. doi: 10.4103/ijdvl.IJDVL_275_17. [Epub ahead of print]

Melanocyte spheroids are formed by repetitive long-term trypsinization.

Author information

1
Department of Dermatology, The Third Affiliated Hospital of Soochow University, Changzhou, China.
2
Department of Dermatology, The First Affiliated Hospital of Bengbu Medical College, Anhui, China.

Abstract

Background:

Autologous melanocyte transplantation plays an important role in the treatment of vitiligo.

Objective:

Previous studies have indicated that, compared with melanocytes growing in monolayers, melanocyte spheroids have a better survival in growth factor- and serum-deprived conditions.

Methods:

Melanocyte spheroids were obtained from human epidermis by repetitive long-term trypsinization and maintained an aggregated morphology for a short period in certain conditions.

Results:

Melanocyte spheroids were capable of growing into normal dendritic melanocytes in monolayer when they were harvested and reinoculated in 24-well plates. Immunohistochemical analysis of the melanocyte spheroids revealed that they were positive for HMB45, a melanosome-specific marker. No melanomas occurred when melanocyte spheroids were transplanted into mice.

Conclusion:

Our study provides a promising approach for melanocyte transplantation to treat vitiligo.

KEYWORDS:

Melanocytes; spheroid; trypsinization

PMID:
30785123
DOI:
10.4103/ijdvl.IJDVL_275_17
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