Format

Send to

Choose Destination
Methods Mol Biol. 2019;1950:283-297. doi: 10.1007/978-1-4939-9139-6_17.

Intranasal Delivery of Adenoviral and AAV Vectors for Transduction of the Mammalian Peripheral Olfactory System.

Author information

1
Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL, USA.
2
Center for Smell and Taste, University of Florida College of Medicine, Gainesville, FL, USA.
3
Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL, USA. martensj@ufl.edu.
4
Center for Smell and Taste, University of Florida College of Medicine, Gainesville, FL, USA. martensj@ufl.edu.

Abstract

Intranasal delivery of solutions is a straightforward methodology for viral vector transduction and gene transfer to the epithelia within the nasal cavity. Beyond the simplicity of the technique, intranasal delivery has demonstrated restricted transduction of the olfactory and respiratory epithelial tissues. Here we outline the procedure of viral vector intranasal delivery in early postnatal and adult mice, as well as adult rats. The procedure allows for robust transduction and ectopic gene delivery that can be used for the visualization of cellular structures, protein distribution, and assessment of viral vector-mediated therapies.

KEYWORDS:

Adeno-associated virus; Adenovirus; Gene therapy; Intranasal delivery; Nasal epithelium; Olfactory epithelium

PMID:
30783981
DOI:
10.1007/978-1-4939-9139-6_17
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center