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Methods Mol Biol. 2019;1950:237-247. doi: 10.1007/978-1-4939-9139-6_13.

Peripheral AAV Injection for Retrograde Transduction of Dorsal Root and Trigeminal Ganglia.

Author information

1
Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL, USA. dbloom@ufl.edu.
2
Department of Obstetrics and Gynecology, University of Colorado School of Medicine, Aurora, CO, USA.
3
Department of Pharmacology and Experimental Therapeutics, Louisiana State University Health Sciences Center, New Orleans, LA, USA.

Abstract

Adeno-associated Virus (AAV) vectors are useful vehicles for delivering transgenes to a number of different tissues and organs in vivo. To date, most of these applications deliver the vectors to their target by either infusion into the bloodstream or direct injection into the target tissue. Recently there has been progress in delivering AAV vectors to neurons of the peripheral nervous system (PNS) following application of vectors to the peripheral epithelium, such as the skin or eye. This delivery only requires treatment of the epithelium to access the underlying nerve termini, and following treatment the vectors are transported retrogradely to the cell bodies of these neurons in the ganglia, such as dorsal root ganglia (DRG) or trigeminal ganglia (TG). Here we describe the methodology for highly efficient transduction of mouse DRG and rabbit TG following application of AAV vectors to the foot, or to the cornea, respectively.

KEYWORDS:

Axonal transport; Gene therapy; Gene transfer; Peripheral nerves; Sensory neurons

PMID:
30783977
DOI:
10.1007/978-1-4939-9139-6_13
[Indexed for MEDLINE]

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