Send to

Choose Destination
Hum Exp Toxicol. 2019 Jun;38(6):619-631. doi: 10.1177/0960327119831067. Epub 2019 Feb 19.

Beneficial role of vanillin, a polyphenolic flavoring agent, on maneb-induced oxidative stress, DNA damage, and liver histological changes in Swiss albino mice.

Author information

1 Animal Physiology Laboratory, Department of Life Sciences, University of Sfax, Sfax, Tunisia.
2 Physiology and Aquatic Environment Unit, Department of Biological Sciences, University of Tunis El Manar, Tunis, Tunisia.
3 Biochemistry Laboratory, Department of Biochemistry, CHU Hedi Chaker, University of Sfax, Sfax, Tunisia.
4 Histopathology Laboratory, Department of Anatomopathology, CHU Habib Bourguiba, University of Sfax, Sfax, Tunisia.


Vanillin, a widely used flavoring agent, has antimutagenic and antioxidant properties. The current study was performed to evaluate its beneficial role against hepatotoxicity induced by maneb, a dithiocarbamate fungicide. Mice were divided into four groups of six each: group 1, serving as negative controls which received by intraperitoneal way only distilled water, a solvent of maneb; group 2, received daily, by intraperitoneal way, maneb (30 mg kg-1 body weight (BW)); group 3, received maneb at the same dose of group 2 and 50 mg kg-1 BW of vanillin by intraperitoneal way; and group 4, serving as positive controls, received daily only vanillin. After 10 days of treatment, mice of all groups were killed. Our results showed that vanillin significantly reduced the elevated hepatic levels of malondialdehyde, hydrogen peroxide, and advanced oxidation protein product and attenuated DNA fragmentation induced by maneb. In addition, vanillin modulated the alterations of antioxidant status: enzymatic (superoxide dismutase, catalase, and glutathione peroxidase) and nonenzymatic (reduced glutathione, nonprotein thiol, and vitamin C) antioxidants in the liver of maneb-treated mice. This natural compound was also able to ameliorate plasma biochemical parameters (aspartate aminotransferase, alanine aminotransferase, gamma glutamyl transpeptidase, alkaline phosphatase, total bilirubin, and total protein). The protective effect of vanillin was further evident through the histopathological changes produced by maneb in the liver tissue. Thus, we concluded that vanillin might be beneficial against maneb-induced hepatic damage in mice.


DNA fragmentation; Vanillin; liver; maneb; oxidative stress


Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center