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J Orthop Surg Res. 2019 Feb 18;14(1):55. doi: 10.1186/s13018-019-1084-2.

High-fat and high-glucose microenvironment decreases Runx2 and TAZ expression and inhibits bone regeneration in the mouse.

Wu X1,2, Zhang Y1,2, Xing Y1,2, Zhao B1,2, Zhou C1,2, Wen Y3,4, Xu X5,6.

Author information

1
Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, 250012, Shandong, People's Republic of China.
2
School of Stomatology, Shandong University, No. 44-1, Wenhua Xi Road, Jinan, China.
3
Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, 250012, Shandong, People's Republic of China. wenyong@sdu.edu.cn.
4
School of Stomatology, Shandong University, No. 44-1, Wenhua Xi Road, Jinan, China. wenyong@sdu.edu.cn.
5
Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, 250012, Shandong, People's Republic of China. xinxu@sdu.edu.cn.
6
School of Stomatology, Shandong University, No. 44-1, Wenhua Xi Road, Jinan, China. xinxu@sdu.edu.cn.

Abstract

BACKGROUND:

Type 2 diabetes mellitus (T2DM) and hyperlipidemia are negatively related to bone regeneration. The aim of this study was to evaluate the effect of high-fat and high-glucose microenvironment on bone regeneration and to detect the expression of runt-related transcription factor 2 (Runx2) and transcriptional co-activator with PDZ-binding domain (TAZ) during this process.

METHODS:

After establishing a high-fat and high-glucose mouse model, a 1 mm × 1.5 mm bone defect was developed in the mandible. On days 7, 14, and 28 after operation, bone regeneration was evaluated by hematoxylin-eosin staining, Masson staining, TRAP staining, and immunohistochemistry, while Runx2 and TAZ expression were detected by immunohistochemistry, RT-PCR, and Western blot analysis.

RESULTS:

Our results showed that the inhibition of bone regeneration in high-fat and high-glucose group was the highest among the four groups. In addition, the expression of Runx2 in high-fat, high-glucose, and high-fat and high-glucose groups was weaker than that in the control group, but the expression of TAZ only showed a decreasing trend in the high-fat and high-glucose group during bone regeneration.

CONCLUSIONS:

In conclusion, these results suggest that high-fat and high-glucose microenvironment inhibits bone regeneration, which may be related to the inhibition of Runx2 and TAZ expression.

KEYWORDS:

Bone regeneration; Hyperlipidemia; Runx2 (runt-related transcription factor 2); T2DM (type 2 diabetes mellitus); TAZ (transcriptional co-activator with PDZ-binding domain)

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