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Methods Mol Biol. 2019;1948:145-156. doi: 10.1007/978-1-4939-9124-2_12.

Yeast-Based Screens to Target Alpha-Synuclein Toxicity.

Author information

1
Department of Experimental Neurodegeneration, Center for Biostructural Imaging of Neurodegeneration, University Medical Center Goettingen, Goettingen, Germany.
2
Department of Molecular Microbiology and Genetics, Institute of Microbiology and Genetics, Georg-August-Universität Göttingen, Göttingen, Germany.
3
Center for Nanoscale Microscopy and Molecular Physiology of the Brain, Goettingen, Germany.
4
Department of Experimental Neurodegeneration, Center for Biostructural Imaging of Neurodegeneration, University Medical Center Goettingen, Goettingen, Germany. touteir@gwdg.de.
5
Center for Nanoscale Microscopy and Molecular Physiology of the Brain, Goettingen, Germany. touteir@gwdg.de.
6
Max Planck Institute for Experimental Medicine, Goettingen, Germany. touteir@gwdg.de.

Abstract

The budding yeast Saccharomyces cerevisiae (S. cerevisiae) has been a remarkable experimental model for the discovery of fundamental biological processes. The high degree of conservation of cellular and molecular processes between the budding yeast and higher eukaryotes has made it a valuable system for the investigation of the molecular mechanisms behind various types of devastating human pathologies. Genetic screens in yeast provided important insight into the toxic mechanisms associated with the accumulation of misfolded proteins. Thus, using yeast genetics and high-throughput screens, novel molecular targets with therapeutic potential have been identified. Here, we describe a yeast screen protocol for the identification of genetic modifiers of alpha-synuclein (aSyn) toxicity, thereby accelerating the identification of novel potential targets for intervention in Parkinson's disease (PD) and other synucleinopathies.

KEYWORDS:

Alpha-synuclein; Genetic screens; Parkinson’s disease; Saccharomyces cerevisiae; Synucleinopathies; Yeast

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