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Vet Comp Oncol. 2019 Feb 15. doi: 10.1111/vco.12469. [Epub ahead of print]

Nectin-4 and p63 immunohistochemical expression in canine prostate tumourigenesis.

Author information

1
Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
2
School of Veterinary Science, The University of Queensland, Brisbane, Queensland, Australia.
3
Department of Veterinary Medicine, University of Bari, Bari, Italy.
4
Department of Veterinary Medicine, Università degli Studi di Milano, Milan, Italy.
5
Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy.

Abstract

Nectin-4 is an E-cadherin-based adherens junction protein of normal epithelial cells, as well as a potent mediator of anchorage-independent cancer colony formation. It is considered a tumour-associated histological and serological marker in various human cancers. The transcription factor p63 is a basal cell marker in the normal prostate, involved in cell adhesion, as well as in the formation and survival of circulating tumour cell clusters. The aim of this study was to evaluate Nectin-4 and p63 immunohistochemical expression in 42 canine prostate tissues including 2 normal prostates, 10 benign prostatic hyperplasias (BPHs), 30 prostatic carcinomas (PCs), 1 pulmonary and 1 lymph node metastasis. From normal to neoplastic tissues, Nectin-4 showed a progressive switching from membranous (m-Nectin-4) to cytoplasmic (c-Nectin-4), regardless of the histological subtypes, except for lack of expression in solid PCs. Metastatic cells exhibited both strong membranous and cytoplasmic positivity. c-Nectin-4 expression was significantly (P < 0.0001) increased in PCs/metastasis compared to BPHs cases and a decrease (P < 0.05) of nuclear p63 immunostaining was also detected in the two groups. Furthermore, data showed a significant association (P < 0.05) between p63 and m-Nectin-4 distribution, although their colocalization was detected only in scattered cells by double immunofluorescence. Our results suggest the involvement of m-Nectin-4 in canine prostate tumourigenesis and metastatic potential, while the exact role of c-Nectin-4 expression detectable in primary PCs requires further investigations.

KEYWORDS:

Nectin-4; cancer; dog; immunohistochemistry; p63; prostate

PMID:
30767361
DOI:
10.1111/vco.12469

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