Format

Send to

Choose Destination
Nat Commun. 2019 Feb 14;10(1):752. doi: 10.1038/s41467-019-08672-6.

Numerous cultivated and uncultivated viruses encode ribosomal proteins.

Author information

1
Unité de Biologie Moléculaire du Gène chez les Extrêmophiles, Département de Microbiologie, Institut Pasteur, Paris, 75015, France.
2
Univ Rennes, CNRS, IGDR (Institut de génétique et développement de Rennes) - UMR 6290, F-35000, Rennes, France.
3
Department of Energy Joint Genome Institute, Walnut Creek, CA, 94598, USA.
4
Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en santé environnement et travail)-UMR_S 1085, PROTIM, F-35000, Rennes, France.
5
Departamento de Producción Vegetal y Microbiología, Evolutionary Genomics Group, Universidad Miguel Hernandez, Alicante, 03550, Spain.
6
Department of Microbiology, The Ohio State University, Columbus, OH, 43210, USA.
7
Department of Civil, Environmental and Geodetic Engineering, The Ohio State University, Columbus, OH, 43210, USA.
8
Unité de Biologie Moléculaire du Gène chez les Extrêmophiles, Département de Microbiologie, Institut Pasteur, Paris, 75015, France. krupovic@pasteur.fr.

Abstract

Viruses modulate ecosystems by directly altering host metabolisms through auxiliary metabolic genes. However, viral genomes are not known to encode the core components of translation machinery, such as ribosomal proteins (RPs). Here, using reference genomes and global-scale viral metagenomic datasets, we identify 14 different RPs across viral genomes arising from cultivated viral isolates and metagenome-assembled viruses. Viruses tend to encode dynamic RPs, easily exchangeable between ribosomes, suggesting these proteins can replace cellular versions in host ribosomes. Functional assays confirm that the two most common virus-encoded RPs, bS21 and bL12, are incorporated into 70S ribosomes when expressed in Escherichia coli. Ecological distribution of virus-encoded RPs suggests some level of ecosystem adaptations as aquatic viruses and viruses of animal-associated bacteria are enriched for different subsets of RPs. Finally, RP genes are under purifying selection and thus likely retained an important function after being horizontally transferred into virus genomes.

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center