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BMC Nephrol. 2019 Feb 12;20(1):49. doi: 10.1186/s12882-019-1221-5.

Magnesium lithospermate B improves renal hemodynamics and reduces renal oxygen consumption in 5/6th renal ablation/infarction rats.

Lin P1,2,3,4, Wu M1,2,3,4, Qin J1,2,3,4, Yang J1,2,3,4, Ye C1,2,3,4, Wang C5,6,7,8.

Author information

1
Department of Nephrology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, No.528 Zhangheng Road, Pudong District, Shanghai, 201203, People's Republic of China.
2
TCM Institute of Kidney Disease, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China.
3
, Key Laboratory of Liver and Kidney Diseases (Shanghai University of Traditional Chinese Medicine) Ministry of Education, Shanghai, People's Republic of China.
4
Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China.
5
Department of Nephrology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, No.528 Zhangheng Road, Pudong District, Shanghai, 201203, People's Republic of China. chenwang8@hotmail.com.
6
TCM Institute of Kidney Disease, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China. chenwang8@hotmail.com.
7
, Key Laboratory of Liver and Kidney Diseases (Shanghai University of Traditional Chinese Medicine) Ministry of Education, Shanghai, People's Republic of China. chenwang8@hotmail.com.
8
Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China. chenwang8@hotmail.com.

Abstract

BACKGROUND:

Magnesium lithospermate B (MLB) can promote renal microcirculation. The aim of the current project was to study whether MLB improves renal hemodynamics, oxygen consumption and subsequently attenuates hypoxia in rats induced by 5/6th renal Ablation/Infarction(A/I).

METHODS:

Chronic renal failure (CRF) was induced in male SD rats by the 5/6 (A/I) surgery. 30 rats were randomly divided into three groups: sham group, 5/6 (A/I) + vehicle group (CRF group) and 5/6 (A/I) + MLB (CRF + MLB) group. 28 days after the surgery, rats were given with saline or 100 mg/kg MLB by i.p. injection for 8 weeks. The 24-h urinary protein (24hUp), serum creatinine (Scr), blood urine nitrogen (BUN), systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured. The protein expression of Fibronectin (FN), Collagen-I (Col-I), Connective Tissue Growth Factor(CTGF) and Interleukin-6 (IL-6) were measured by Western blot. Renal blood flow (RBF) and renal O2 consumption (QO2) indicated as sodium reabsorption (QO2/TNa) were detected before sacrifice. Renal hypoxia was assessed by measuring the protein expression of nNOS, HIF-1α and VEGF.

RESULTS:

MLB significantly reduced 24hUp, Scr, BUN, SBP and DBP levels in rats with CRF. The expression of FN, Col-I, CTGF and IL-6 were down-regulated by MLB treatment in rats with CRF. In comparison to sham operated rats, 5/6 (A/I) rats had significantly lower RBF, and MLB significantly increased RBF in rats with CRF. Moreover, QO2/TNa was higher in the CRF group as compared to that in the sham group, and it was significantly attenuated in the CRF + MLB group. MLB reversed the expression of nNOS (neuronal nitric oxide synthase), HIF-1α (hypoxia inducible factor-1) and VEGF in rats with CRF.

CONCLUSIONS:

MLB improves renal function, fibrosis and inflammation in CRF rats induced by 5/6 (A/I), which is probably related to the increase in RBF, reduction of oxygen consumption and attenuation of renal hypoxia in the remnant kidney with CRF.

KEYWORDS:

CRF; Hypoxia; Magnesium lithospermate B; Renal blood flow; Renal oxygen consumption

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