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Plant Cell Rep. 2000 Apr;19(5):472-477. doi: 10.1007/s002990050758.

Establishment and characteristics of an anthocyanin-producing cell line from sweet potato storage root.

Author information

1
Laboratory of Upland Crop Genetic Resources, Department of Upland Farming, Kyushu National Agricultural Experiment Station, 6644 Yokoichi, Miyakonojo, Miyazaki 885-0091, Japan e-mail: mnakatan@mykz.affrc.go.jp, , , , , , JP.
2
Department of Food Science and Technology, College of Horticulture, Minami-Kyushu University, Takanabe, Miyazaki 884-0003, Japan, , , , , , JP.

Abstract

 Anthocyanin pigments accumulated in a cell line derived from storage-root explants of sweet potato (Ipomoea batatas L.) cv 'Ayamurasaki'. Somatic pro-embryos were induced on the explants cultured on Murashige and Skoog medium supplemented with 1 mg/l 2,4-D. The pro-embryo structures produced callus when transferred to MS medium with 0.5 mg/l 2,4-D. A cell line was isolated from this callus which accumulated anthocyanin pigment. The color value of the pigment extracted after 27 days of culture in MS medium with 2 mg/l 2,4-D was 8.2, which was very close to that of a pigment extracted from roots, which was 8.9. Most of the pigments from the cell extract were hydrophilic and appeared on the ODS-column HPLC with a lower retention time than the main anthocyanins of the root tissues. The majority of the pigments were identical with the root anthocyanins. Cell line-specific anthocyanins were detected.

KEYWORDS:

Anthocyanin composition; Callus culture; Key words Ipomoea batatas

PMID:
30754885
DOI:
10.1007/s002990050758

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