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JCI Insight. 2019 Mar 21;4(6). pii: 123672. doi: 10.1172/jci.insight.123672. eCollection 2019 Mar 21.

Systematic testing and specificity mapping of alloantigen-specific chimeric antigen receptors in regulatory T cells.

Author information

1
Department of Medicine and.
2
BC Children's Hospital Research Institute (BCCHR), Vancouver, British Columbia, Canada.
3
Department of Surgery, University of British Columbia (UBC), Vancouver, British Columbia, Canada.
4
Centre for Drug and Research and Development, Vancouver, British Columbia, Canada.
5
Department of Pathology and Laboratory Medicine and.
6
School of Biomedical Engineering, UBC, Vancouver, British Columbia, Canada.

Abstract

Chimeric antigen receptor (CAR) technology can be used to engineer the antigen specificity of regulatory T cells (Tregs) and improve their potency as an adoptive cell therapy in multiple disease models. As synthetic receptors, CARs carry the risk of immunogenicity, particularly when derived from nonhuman antibodies. Using an HLA-A*02:01-specific CAR (A2-CAR) encoding a single-chain variable fragment (Fv) derived from a mouse antibody, we developed a panel of 20 humanized A2-CARs (hA2-CARs). Systematic testing demonstrated variations in expression, and ability to bind HLA-A*02:01 and stimulate human Treg suppression in vitro. In addition, we developed a new method to comprehensively map the alloantigen specificity of CARs, revealing that humanization reduced HLA-A cross-reactivity. In vivo bioluminescence imaging showed rapid trafficking and persistence of hA2-CAR Tregs in A2-expressing allografts, with eventual migration to draining lymph nodes. Adoptive transfer of hA2-CAR Tregs suppressed HLA-A2+ cell-mediated xenogeneic graft-versus-host disease and diminished rejection of human HLA-A2+ skin allografts. These data provide a platform for systematic development and specificity testing of humanized alloantigen-specific CARs that can be used to engineer specificity and homing of therapeutic Tregs.

KEYWORDS:

Immunology; Immunotherapy; Organ transplantation; Tolerance; Transplantation

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