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Int J Syst Evol Microbiol. 2019 Apr;69(4):1093-1098. doi: 10.1099/ijsem.0.003277. Epub 2019 Feb 12.

Salinibacterium hongtaonis sp. nov., isolated from faeces of Tibetan antelope (Pantholops hodgsonii) on the Qinghai-Tibet Plateau.

Li J1, Lu S2,3, Jin D2,3, Yang J2,3, Lai XH4, Zhang G2, Tian Z1, Zhu W3, Pu J2, Wu X5, Huang Y2, Wang S1, Xu J2,1.

Author information

1
1​Department of Epidemiology, Shanxi Medical University School of Public Health, Taiyuan, Shanxi 030001, PR China.
2
2​State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.
3
3​Shanghai Institute for Emerging and Re-emerging infectious diseases, Shanghai Public Health Clinical Center, Shanghai, 201508, PR China.
4
4​School of Biology and Food Sciences, Shangqiu Normal University, Henan province, 475000, PR China.
5
5​Shaanxi Institute of Zoology, Xi'an, Shaanxi Province, 710032, PR China.

Abstract

Two novel aerobic, Gram-staining-positive and non-spore-forming bacterial strains, 194T and S1194, were isolated from faeces of Tibetan antelopes sampled at the Qinghai-Tibet Plateau of China. The strains were able to grow in medium up to 10 % NaCl, similar to the NaCl-resistant property of the genus Salinibacterium members. The 16S rRNA gene sequences of the strains showed the highest similarity to Salinibacterium xinjiangense(98.1-98.2 %), and phylogenetic analysis based on 16S rRNA gene sequences indicated that strains 194T and S1194 represent a new lineage. The DNA G+C contents of strain 194T and S1194 are 64.1 and 64.2 mol%. Their genomes exhibit less than 96 % average nucleotide identity and 70 % DNA-DNA relatedness to known species of Salinibacterium. Strains 194T and S1194 are unable to utilize d-mannose or produce naphthol-AS-BI-phosphohydrolase. The two strains had anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids, and their cell walls contained lysine, alanine, glycine and glutamic acid. The predominant menaquinones identified were MK-11 and MK-10, with diphosphatidylglycerol and phosphatidylglycerol as major polar lipids. Overall, the major cellular content profiles of 194T agreed with those of Salinibacterium xinjiangense and Salinibacterium amurskyense, though the proportions were distinct. Based on genotypic, phenotypic and biochemical analyses, the novel species Salinibacterium hongtaonis sp. nov. is proposed. The type strain is 194T (=CGMCC 1.16371T=DSM 106171T).

KEYWORDS:

Qinghai-Tibet Plateau; Salinibacterium; Tibetan antelope; novel species

PMID:
30747615
DOI:
10.1099/ijsem.0.003277
[Indexed for MEDLINE]

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