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J Mater Sci Mater Med. 2019 Feb 12;30(2):28. doi: 10.1007/s10856-019-6230-1.

Micropatterning of endothelial cells to create a capillary-like network with defined architecture by laser-assisted bioprinting.

Author information

1
INSERM, Bioingénierie Tissulaire, U1026, 146 rue Léo Saignat, F-33076, Bordeaux, France. olivia.kerouredan@u-bordeaux.fr.
2
Université de Bordeaux, Bioingénierie Tissulaire, U1026, 146 rue Léo Saignat, F-33076, Bordeaux, France. olivia.kerouredan@u-bordeaux.fr.
3
CHU de Bordeaux, Services d'Odontologie et de Santé Buccale, Place Amélie Raba Léon, F-33076, Bordeaux, France. olivia.kerouredan@u-bordeaux.fr.
4
INSERM, Bioingénierie Tissulaire, U1026, 146 rue Léo Saignat, F-33076, Bordeaux, France.
5
Energie, matériaux et télécommunication, Institut National de Recherche Scientifique, Varenne, QC, Canada.
6
Université de Bordeaux, Bioingénierie Tissulaire, U1026, 146 rue Léo Saignat, F-33076, Bordeaux, France.
7
Université de Bordeaux, ARNA Laboratory, team ChemBioPharm, U1212 INSERM - UMR 5320 CNRS, 146 rue Léo Saignat, F-33076, Bordeaux, France.
8
CHU de Bordeaux, Pharmacie du Groupe Hospitalier Sud, Avenue de Magellan, F-33604, Pessac, France.
9
CHU de Bordeaux, Services d'Odontologie et de Santé Buccale, Place Amélie Raba Léon, F-33076, Bordeaux, France.

Abstract

Development of a microvasculature into tissue-engineered bone substitutes represents a current challenge. Seeding of endothelial cells in an appropriate environment can give rise to a capillary-like network to enhance prevascularization of bone substitutes. Advances in biofabrication techniques, such as bioprinting, could allow to precisely define a pattern of endothelial cells onto a biomaterial suitable for in vivo applications. The aim of this study was to produce a microvascular network following a defined pattern and preserve it while preparing the surface to print another layer of endothelial cells. We first optimise the bioink cell concentration and laser printing parameters and then develop a method to allow endothelial cells to survive between two collagen layers. Laser-assisted bioprinting (LAB) was used to pattern lines of tdTomato-labeled endothelial cells cocultured with mesenchymal stem cells seeded onto a collagen hydrogel. Formation of capillary-like structures was dependent on a sufficient local density of endothelial cells. Overlay of the pattern with collagen I hydrogel containing vascular endothelial growth factor (VEGF) allowed capillary-like structures formation and preservation of the printed pattern over time. Results indicate that laser-assisted bioprinting is a valuable technique to pre-organize endothelial cells into high cell density pattern in order to create a vascular network with defined architecture in tissue-engineered constructs based on collagen hydrogel.

PMID:
30747358
DOI:
10.1007/s10856-019-6230-1
[Indexed for MEDLINE]

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