Format

Send to

Choose Destination
Curr Biol. 2019 Feb 18;29(4):605-615.e6. doi: 10.1016/j.cub.2019.01.045. Epub 2019 Feb 7.

Estrus-Cycle Regulation of Cortical Inhibition.

Author information

1
Bernstein Center for Computational Neuroscience Berlin, Humboldt-Universität zu Berlin, Philippstraße 13, Haus 6, 10115 Berlin, Germany.
2
Champalimaud Center for the Unknown, Neurosciences, Avenida Brasília, 1400-038 Lisbon, Portugal.
3
Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health; Neuroscience Research Center, NeuroCure Cluster of Excellence, Charitéplatz 1, 10117 Berlin, Germany.
4
Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Xueyuan Boulevard, 518055 Shenzhen, China.
5
Bernstein Center for Computational Neuroscience Berlin, Humboldt-Universität zu Berlin, Philippstraße 13, Haus 6, 10115 Berlin, Germany; Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health; Neuroscience Research Center, NeuroCure Cluster of Excellence, Charitéplatz 1, 10117 Berlin, Germany. Electronic address: michael.brecht@bccn-berlin.de.

Abstract

Female mammals experience cyclical changes in sexual receptivity known as the estrus cycle. Little is known about how estrus affects the cortex, although alterations in sensation, cognition and the cyclical occurrence of epilepsy suggest brain-wide processing changes. We performed in vivo juxtacellular and whole-cell recordings in somatosensory cortex of female rats and found that the estrus cycle potently altered cortical inhibition. Fast-spiking interneurons were strongly activated with social facial touch and varied their ongoing activity with the estrus cycle and estradiol in ovariectomized females, while regular-spiking excitatory neurons did not change. In situ hybridization for estrogen receptor β (Esr2) showed co-localization with parvalbumin-positive (PV+) interneurons in deep cortical layers, mirroring the laminar distribution of our physiological findings. The fraction of neurons positive for estrogen receptor β (Esr2) and PV co-localization (Esr2+PV+) in cortical layer V was increased in proestrus. In vivo and in vitro experiments confirmed that estrogen acts locally to increase fast-spiking interneuron excitability through an estrogen-receptor-β-dependent mechanism.

KEYWORDS:

cortex; estrus; female; inhibition; interneurons; parvalbumin; rat; social touch

PMID:
30744972
DOI:
10.1016/j.cub.2019.01.045

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center