Format

Send to

Choose Destination
Phytochemistry. 2019 Feb 8;160:78-84. doi: 10.1016/j.phytochem.2019.01.013. [Epub ahead of print]

Steroidal glycosides from Ornithogalum dubium Houtt.

Author information

1
Laboratoire de Pharmacognosie, PEPITE EA 4267, UFR des Sciences de Santé, Université de Bourgogne Franche-Comté, 21079, Dijon Cedex, France.
2
Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, 812-8582, Japan.
3
Universität Siegen, OC-II, Naturwissenschaftlich-Technische Fakultät, D-57068, Siegen, Germany.
4
Université de Bourgogne, Centre Hospitalier Universitaire de Dijon, INSERM, U1231, Lipides Nutrition Cancer, Équipe labellisée Ligue Nationale contre le Cancer, Dijon, France.
5
Laboratoire de Pharmacognosie, PEPITE EA 4267, UFR des Sciences de Santé, Université de Bourgogne Franche-Comté, 21079, Dijon Cedex, France. Electronic address: m-a.lacaille-dubois@u-bourgogne.fr.

Abstract

The phytochemical study of Ornithogalum dubium Houtt. (Asparagaceae) led to the isolation of five undescribed steroidal glycosides together with two known ones. Their structures were established by using NMR analysis and mass spectrometry as (25R)-3β-hydroxyspirost-5-en-1β-yl O-α-L-arabinopyranosyl-(1 → 2)-α-L-rhamnopyranoside, (25S)-3β-hydroxyspirost-5-en-1β-yl O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranoside, (22S)-16β-[(α-L-rhamnopyranosyl)oxy]-22-hydroxycholest-5-en-3β-yl O-β-D-glucopyranosyl-(1 → 4)-β-D-glucopyranoside, (22S,23S)-1β,3β,11α,16β,23-pentahydroxy-5α-cholest-24-en-22β-yl β-D-glucopyranoside, (22S,23S)-3β-[(β-D-glucopyranosyl)oxy]-22,23-dihydroxy-5α-cholest-24-en-16β-yl O-α-L-rhamnopyranosyl)-(1 → 4)-β-D-glucopyranoside. Their cytotoxic activities against two human cells, a lung carcinoma A-549 and a promyelocytic leukemia HL-60 cell lines, were evaluated by using the XTT method. The results showed no significant cytotoxicity on the tested cells. The influence of the potentiation of cisplatin cytotoxicity in A-549 cells was also investigated and a slight effect was observed only for the (25R) spirostane-type derivative.

KEYWORDS:

Asparagaceae; Cholestane glycosides; Cytotoxicity; NMR; Ornithogalum dubium; Spirostane glycosides

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center