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J Agric Food Chem. 2019 Feb 20;67(7):1831-1838. doi: 10.1021/acs.jafc.8b05481. Epub 2019 Feb 11.

Neuroprotective Secondary Metabolite Produced by an Endophytic Fungus, Neosartorya fischeri JS0553, Isolated from Glehnia littoralis.

Author information

1
College of Pharmacy and Innovative Drug Center , Duksung Women's University , Seoul 01369 , Republic of Korea.
2
College of Korean Medicine , Gachon University , Seongnam 13120 , Republic of Korea.
3
Biological Resources Assessment Division , National Institute of Biological Resources , Incheon 22689 , Republic of Korea.
4
Department of Food Science and Biotechnology, College of Life Science , CHA University , Pocheon 13488 , Republic of Korea.

Abstract

Roots of Glehnia littoralis have been used to heal stroke as a traditional medicine. Even though many studies on this plant have been conducted, the secondary metabolites produced by its endophytes and their bioactivities have not been investigated thus far. Therefore, a new meroditerpenoid named sartorypyrone E (1) and eight known compounds (2-9) were isolated from extracts of cultured Neosartorya fischeri JS0553, an endophyte of G. littoralis. The isolated metabolites were identified using spectroscopic methods and chemical reaction, based on a comparison to literature data. Relative and absolute stereochemistries of compound 1 were also elucidated. To identify the protective effects of isolated compounds (1-9) in HT22 cells against glutamate-induced cytotoxicity, we assessed inhibition of cell death, intracellular reactive oxygen species (ROS) accumulation, and calcium ion (Ca2+) influx. Among the isolates, compound 8, identified as fischerin, showed significant neuroprotective activity on glutamate-mediated HT22 cell death through inhibition of ROS, Ca2+ influx, and phosphorylation of mitogen-activated protein kinase, including c-Jun N-terminal kinase, extracellular signal-regulated kinase, and p38. The results suggested that the metabolites produced by the endophyte N. fischeri JS0553 might be related to the neuroprotective activity of its host plant, G. littoralis.

KEYWORDS:

Neosartorya fischeri; endophyte; neuroprotection; sartorypyrone E

PMID:
30742443
DOI:
10.1021/acs.jafc.8b05481
[Indexed for MEDLINE]

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