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Sci Rep. 2019 Feb 11;9(1):1746. doi: 10.1038/s41598-018-36089-6.

Bioactive glycoalkaloides isolated from Solanum melongena fruit peels with potential anticancer properties against hepatocellular carcinoma cells.

Author information

1
Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
2
Biomedical Research Section, Nawah Scientific, Mokattam, Cairo, Egypt.
3
Department of Pharmacognosy, Faculty of Pharmacy, October University for Modern Science and Arts (MSA), 6th October, Giza, 12566, Egypt.
4
Pharmacognosy Department, Faculty of Pharmacy, The British University of Egypt, El-Sherouk city, Cairo, 11837, Cairo, Egypt.
5
Research Centre, King Faisal Specialist Hospital and Research Center, Jeddah, 21499, Saudi Arabia.
6
Pharmacology Department, Medical Division, National Research Centre, Dokki, Giza, Egypt. ahmedmalabd@pharma.asu.edu.eg.
7
Department of Pharmaceutical Sciences, College of Pharmacy, Gulf Medical University, Ajman, 4184, Ajman, United Arab Emirates. ahmedmalabd@pharma.asu.edu.eg.

Abstract

Hepatocellular carcinoma (HCC) is progressively increasing tumor with lack of accurate prognosis and inadequate systemic treatment approaches. Solanum sp. (such as Solanum melongena) is a folk herb which is reported to possess anticancer properties. In a continuity for our interest in pursuing the anticancer activity of compounds isolated from the fruit peels of Solanum melongena, the HPLC profiling and ESI-MS assessment for the methanolic extract evidenced the presence of bioactive glycoalkaloids (solasonine, solasodine and solamargine). These glycoalkaloids were isolated, purified and proved to possess in vitro cytotoxicity against human liver cancer cell lines (Huh7 and HepG2). Herein, we investigated the potential mechanism of action of these compounds using DNA content flow-cytometry and apoptosis/necrosis differential anaylsis using annexin-V/FITC staining. Solasonine, solasodine and solamargine inducd significant antiproliferative effect against liver cancer cells (Huh7 and HepG2) which was attributed to cell cycle arrest at S-phase. Solamargine, solasodine and solasonine induced significant apoptosis in Huh7 cells. Only solamargine-induced cell cycle arrest, was reflected as apoptotic cell killing effect against HepG2 cells. In conclusion, glycoalkaloids derived from Solanum melongena and particularly, solamargine are promising antiproliferative agents with potential anticancer effects.

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