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Biosci Biotechnol Biochem. 2019 Jun;83(6):1146-1156. doi: 10.1080/09168451.2019.1580559. Epub 2019 Feb 11.

Orally administered collagen peptide protects against UVB-induced skin aging through the absorption of dipeptide forms, Gly-Pro and Pro-Hyp.

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a Research & Development Center , Nong Shim Co., Ltd ., Seoul , Republic of Korea.
b Vital Beautie Research Institute , Amorepacific Corporation R&D Center , Yongin-si , Republic of Korea.
c Medical Beauty QA Team , Aestura Corporation , Anseong-si , Republic of Korea.
d Department of Food Science and Biotechnology , College of Life Science and Biotechnology, Dongguk University-Seoul , Goyang , Republic of Korea.


Collagen hydrolysate is a well-known nutritional supplement for the improvement of healthy skin. Here, collagen peptide NS (CPNS) from fish scale was prepared, and its physicochemical properties were investigated. Gly-Pro was revealed as a representative low molecular weight peptide of CPNS, by performing prep-HPLC and LC-MS/MS. CPNS treatment attenuated matrix metalloproteinase-1 production and increased the synthesis of type 1 procollagen in HDF cells. After orally administering CPNS to rats, the plasma concentrations of Gly-Pro and Pro-Hyp increased dramatically. To examine the protective effects of CPNS against ultraviolet B (UVB)-induced photoaging in vivo, the dorsal skins of hairless mice were exposed to UVB and supplemented with CPNS for 12 weeks. The CPNS consumption significantly attenuated UVB-induced wrinkle formation, transepidermal water loss, and epidermis thickness, and increased skin hydration. Collectively, these results suggest that bioactive peptides of CPNS, Gly-Pro and Pro-Hyp, exert beneficial effects on skin health.


Collagen hydrolysate; Gly-Pro; Pro-Hyp; bioactive peptide; skin health

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