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Mol Immunol. 2019 Apr;108:1-7. doi: 10.1016/j.molimm.2019.02.003. Epub 2019 Feb 8.

Fli-1 transcription factor regulates the expression of caspase-1 in lung pericytes.

Author information

1
Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC, 29425, United States.
2
Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, Department of Medicine, Medical University of South Carolina, Charleston, SC, 29425, United States.
3
Department of Neurosciences, Medical University of South Carolina, Charleston, SC, 29425, United States.
4
Department of Medicine, Medical University of South Carolina, Charleston, SC, 29425, United States; Department of Cell and Molecular Pharmacology, Medical University of South Carolina, Charleston, SC, 29425, United States.
5
Division of Rheumatology and Immunology, Department of Medicine, Medical University of South Carolina, Charleston, SC, 29425, United States.
6
Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC, 29425, United States; Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, 29425, United States. Electronic address: fanhong@musc.edu.

Abstract

Our previous data demonstrated that Friend leukemia virus integration 1 (Fli-1), an ETS transcription factor, governs pericyte loss and vascular dysfunction in cecal ligation and puncture-induced murine sepsis by regulating essential pyroptosis markers including caspase-1. However, whether Fli-1 regulates caspase-1 expression levels in vitro and how Fli-1 regulates caspase-1 remain unknown. Our present work further demonstrated that overexpressed Fli-1 significantly increased caspase-1 and IL-18 expression levels in cultured mouse lung pericytes. Bacterial outer membrane vesicles (OMVs) have been found to induce cell pyroptosis through transferring LPS intracellularly. Using OMVs to induce an in vitro model of pyroptosis, we observed that OMVs significantly increased protein levels of Fli-1 in mouse lung pericytes. Furthermore, knockdown of Fli-1 by siRNA blocked OMVs-induced caspase-1, caspase-11 and IL-18 expression levels. As caspase-1 was predicted as a potential target of Fli-1, we cloned murine caspase-1 promoter into a luciferase construct. Our data demonstrate for the first time that Fli-1 regulates caspase-1 expression by directly binding to its promoter regions measured by chromatin immunoprecipitation (ChIP) assay and luciferase reporter system. In summary, our findings demonstrated a novel role and mechanism of Fli-1 in regulating caspase-1 expression in lung pericytes.

KEYWORDS:

Caspase-1; Fli-1; Pericytes

PMID:
30739075
PMCID:
PMC6409192
[Available on 2020-04-01]
DOI:
10.1016/j.molimm.2019.02.003
[Indexed for MEDLINE]

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