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Methods Mol Biol. 2019;1920:183-218. doi: 10.1007/978-1-4939-9009-2_12.

Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections.

Author information

1
EMBL Australia, Australian Regenerative Medicine Institute, Monash University, Clayton, VIC, Australia.
2
Department of Biology, Barnard College, Columbia University, New York, NY, USA.
3
Department of Biology, Barnard College, Columbia University, New York, NY, USA. jmansfield@barnard.edu.

Abstract

Analysis of gene (mRNA and protein) expression patterns is central to the study of embryonic development. This chapter details methods for detecting mRNA and protein expression in whole-mouse embryos and in tissue sections, including mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters. We focus on histological methods; molecular methods of measuring gene expression (for example, RNAseq, PCR) are not included here.

KEYWORDS:

Alkaline phosphatase; Green fluorescent protein; Immunohistochemistry; In situ hybridization; Mouse embryogenesis; ß-Galactosidase

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