Format

Send to

Choose Destination
Methods Mol Biol. 2019;1920:129-141. doi: 10.1007/978-1-4939-9009-2_9.

RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq).

Author information

1
Berlin Institute for Medical Systems Biology, MDC Berlin, Berlin, Germany.
2
Berlin Institute for Medical Systems Biology, MDC Berlin, Berlin, Germany. janphilipp.junker@mdc-berlin.de.

Abstract

Embryonic development is heavily dependent on temporally and spatially restricted gene expression. Spatially resolved measurements of gene expression are therefore crucial for identifying novel regulators and the understanding of their function. However, in situ methods do not resolve global gene expression, and sequencing-based methods usually do not provide spatial information. Here, we describe tomo-seq, a method that combines classical histological sectioning of embryos or tissues with a highly sensitive RNA-sequencing technique. Application of tomo-seq to zebrafish embryos allows reconstructing the spatial gene expression of thousands of genes.

KEYWORDS:

Developmental patterning; Gene expression mapping; Local gene expression; Morphogen gradients; Spatial transcriptomics; mRNA sequencing

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center