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Nat Plants. 2019 Feb;5(2):184-193. doi: 10.1038/s41477-019-0358-3. Epub 2019 Feb 8.

Concerted expression of a cell cycle regulator and a metabolic enzyme from a bicistronic transcript in plants.

Author information

1
Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland. laura.lorenzo@unige.ch.
2
Friedrich Miescher Laboratory of the Max Planck Society, Tübingen, Germany.
3
Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
4
Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland.
5
Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland.
6
Max Planck Institute for Biology of Ageing, Cologne, Germany.
7
Institute of Biostatistics, Leibniz University, Hannover, Germany.
8
Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, Lyon, France.
9
Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland. michael.hothorn@unige.ch.
10
Friedrich Miescher Laboratory of the Max Planck Society, Tübingen, Germany. michael.hothorn@unige.ch.

Abstract

Eukaryotic mRNAs frequently contain upstream open reading frames (uORFs), encoding small peptides that may control translation of the main ORF (mORF). Here, we report the characterization of a distinct bicistronic transcript in Arabidopsis. We analysed loss-of-function phenotypes of the inorganic polyphosphatase TRIPHOSPHATE TUNNEL METALLOENZYME 3 (AtTTM3), and found that catalytically inactive versions of the enzyme could fully complement embryo and growth-related phenotypes. We could rationalize these puzzling findings by characterizing a uORF in the AtTTM3 locus encoding CELL DIVISION CYCLE PROTEIN 26 (CDC26), an orthologue of the cell cycle regulator. We demonstrate that AtCDC26 is part of the plant anaphase promoting complex/cyclosome (APC/C), regulates accumulation of APC/C target proteins and controls cell division, growth and embryo development. AtCDC26 and AtTTM3 are translated from a single transcript conserved across the plant lineage. While there is no apparent biochemical connection between the two gene products, AtTTM3 coordinates AtCDC26 translation by recruiting the transcript into polysomes. Our work highlights that uORFs may encode functional proteins in plant genomes.

PMID:
30737513
DOI:
10.1038/s41477-019-0358-3

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