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Sci Rep. 2019 Feb 8;9(1):1709. doi: 10.1038/s41598-018-38175-1.

Plasmodium falciparum histidine-rich protein (PfHRP2 and 3) diversity in Western and Coastal Kenya.

Author information

1
Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany.
2
School of Health Sciences, Kirinyaga University, Kerugoya, Kenya.
3
Center for Biotechnology Research and Development, Kenya Medical Research Institute, Nairobi, Kenya.
4
Department of Biochemistry and Biotechnology, School of Biological and Life Sciences, Technical University of Kenya, Nairobi, Kenya.
5
Vietnamese-German Centre for Medical Research (VG-CARE), Hanoi, Vietnam.
6
Faculty of Medicine, Duy Tan University, Da Nang, Vietnam.
7
Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany. velavan@medizin.uni-tuebingen.de.
8
Vietnamese-German Centre for Medical Research (VG-CARE), Hanoi, Vietnam. velavan@medizin.uni-tuebingen.de.
9
Faculty of Medicine, Duy Tan University, Da Nang, Vietnam. velavan@medizin.uni-tuebingen.de.
10
Fondation Congolaise pour la Recherche Médicale, Brazzaville, Congo. velavan@medizin.uni-tuebingen.de.

Abstract

Plasmodium falciparum histidine-rich proteins 2 (PfHRP2) based RDTs are advocated in falciparum malaria-endemic regions, particularly when quality microscopy is not available. However, diversity and any deletion in the pfhrp2 and pfhrp3 genes can affect the performance of PfHRP2-based RDTs. A total of 400 samples collected from uncomplicated malaria cases from Kenya were investigated for the amino acid repeat profiles in exon 2 of pfhrp2 and pfhrp3 genes. In addition, PfHRP2 levels were measured in 96 individuals with uncomplicated malaria. We observed a unique distribution pattern of amino acid repeats both in the PfHRP2 and PfHRP3. 228 PfHRP2 and 124 PfHRP3 different amino acid sequences were identified. Of this, 214 (94%) PfHRP2 and 81 (65%) PfHRP3 amino acid sequences occurred only once. Thirty-nine new PfHRP2 and 20 new PfHRP3 amino acid repeat types were identified. PfHRP2 levels were not correlated with parasitemia or the number of PfHRP2 repeat types. This study shows the variability of PfHRP2, PfHRP3 and PfHRP2 concentration among uncomplicated malaria cases. These findings will be useful to understand the performance of PfHRP2-based RDTs in Kenya.

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