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Int J Mol Sci. 2019 Feb 7;20(3). pii: E708. doi: 10.3390/ijms20030708.

Anti-Inflammatory Activity of A Polyphenolic Extract from Arabidopsis thaliana in In Vitro and In Vivo Models of Alzheimer's Disease.

Author information

1
Department of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00185 Roma, Italy. roberto.mattioli@uniroma1.it.
2
Department of Biochemical Sciences, Sapienza University of Rome, 00185 Roma, Italy. antonio.francioso@uniroma1.it.
3
Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany. antonio.francioso@uniroma1.it.
4
Department of Biochemical Sciences, Sapienza University of Rome, 00185 Roma, Italy. maria.derme@uniroma1.it.
5
Department of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00185 Roma, Italy. maurizio.trovato@uniroma1.it.
6
Department of Experimental Medicine, Sapienza University of Rome, 00185 Roma, Italy. patrizia.mancini@uniroma1.it.
7
Department of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00185 Roma, Italy. lucia.piacentini@uniroma1.it.
8
Department of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00185 Roma, Italy. assuntamaria.casale@uniroma1.it.
9
Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany. Ludger.Wessjohann@ipb-halle.de.
10
Department of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00185 Roma, Italy. robertagazzino@gmail.com.
11
Department of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00185 Roma, Italy. paolo.costantino@uniroma1.it.
12
Department of Biochemical Sciences, Sapienza University of Rome, 00185 Roma, Italy. luciana.mosca@uniroma1.it.

Abstract

Alzheimer's disease (AD) is the most common neurodegenerative disorder and the primary form of dementia in the elderly. One of the main features of AD is the increase in amyloid-beta (Aβ) peptide production and aggregation, leading to oxidative stress, neuroinflammation and neurodegeneration. Polyphenols are well known for their antioxidant, anti-inflammatory and neuroprotective effects and have been proposed as possible therapeutic agents against AD. Here, we investigated the effects of a polyphenolic extract of Arabidopsis thaliana (a plant belonging to the Brassicaceae family) on inflammatory response induced by Aβ. BV2 murine microglia cells treated with both Aβ25⁻35 peptide and extract showed a lower pro-inflammatory (IL-6, IL-1β, TNF-α) and a higher anti-inflammatory (IL-4, IL-10, IL-13) cytokine production compared to cells treated with Aβ only. The activation of the Nrf2-antioxidant response element signaling pathway in treated cells resulted in the upregulation of heme oxygenase-1 mRNA and in an increase of NAD(P)H:quinone oxidoreductase 1 activity. To establish whether the extract is also effective against Aβ-induced neurotoxicity in vivo, we evaluated its effect on the impaired climbing ability of AD Drosophila flies expressing human Aβ1⁻42. Arabidopsis extract significantly restored the locomotor activity of these flies, thus confirming its neuroprotective effects also in vivo. These results point to a protective effect of the Arabidopsis extract in AD, and prompt its use as a model in studying the impact of complex mixtures derived from plant-based food on neurodegenerative diseases.

KEYWORDS:

Alzheimer’s disease; Arabidopsis thaliana; BV2 cells; NF-κB; Nrf2; amyloid beta; interleukins; polyphenols

PMID:
30736391
DOI:
10.3390/ijms20030708
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